Anti-OAT3/Slc22a8 Antibody Picoband™

Product Name

Anti-OAT3/Slc22a8 Antibody Picoband™

View all SLC22A8 Antibodies

SKU/Catalog Number

A04087-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-OAT3/Slc22a8 Antibody Picoband™ catalog # A04087-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-OAT3/Slc22a8 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04087-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse OAT3/Slc22a8 recombinant protein (Position: K69-T534).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04087-1 is reactive to SLC22A8 in Mouse

Applications

A04087-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

59.856kDa

Background of SLC22A8

Solute carrier family 22 member 8, or organic anion transporter 3 (OAT3), is a protein that in humans is encoded by the SLC22A8 gene. This gene encodes a protein involved in the sodium-independent transport and excretion of organic anions, some of which are potentially toxic. The encoded protein is an integral membrane protein and appears to be localized to the basolateral membrane of the kidney. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Mouse
Flow Cytometry, 1-3μg/1x10⁶ cells, Mouse,
Direct ELISA, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of OAT3/Slc22a8 using anti-OAT3/Slc22a8 antibody (A04087-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OAT3/Slc22a8 antigen affinity purified polyclonal antibody (Catalog # A04087-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OAT3/Slc22a8 at approximately 60KD. The expected band size for OAT3/Slc22a8 is at 60KD.

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Figure 2. Flow Cytometry analysis of Neuro-2a cells using anti-OAT3/Slc22a8 antibody (A04087-1).
Overlay histogram showing Neuro-2a cells stained with A04087-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OAT3/Slc22a8 Antibody (A04087-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SLC22A8 (Source: Uniprot.org, NCBI)

Alternative Names

hOAT3; MGC24086; OAT3Organic anion transporter 3; solute carrier family 22 (organic anion transporter), member 8; solute carrier family 22 member 8 SLC22A8 OAT3 solute carrier family 22 member 8 solute carrier family 22 member 8|hOAT3|organic anion transporter 3|solute carrier family 22 (organic anion transporter), member 8

For more info on SLC22A8, check out the SLC22A8 Infographic

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In this infographic, you will see the following information for SLC22A8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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