Anti-OB Cadherin/CDH11 Antibody Picoband™

Product Name

Anti-OB Cadherin/CDH11 Antibody Picoband™

View all Cadherin-11 Antibodies

SKU/Catalog Number

A04419-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-OB Cadherin/CDH11 Antibody Picoband™ catalog # A04419-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-OB Cadherin/CDH11 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04419-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human OB Cadherin/CDH11 recombinant protein (Position: K371-S796).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04419-1 is reactive to CDH11 in Human, Mouse, Rat

Applications

A04419-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

120 kDa

Calculated molecular weight

87.965kDa

Background of Cadherin-11

Cadherin-11 is a protein that in humans is encoded by the CDH11 gene. This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat
Flow Cytometry, 1-3μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of OB Cadherin/CDH11 using anti-OB Cadherin/CDH11 antibody (A04419-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OB Cadherin/CDH11 antigen affinity purified polyclonal antibody (Catalog # A04419-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OB Cadherin/CDH11 at approximately 120KD. The expected band size for OB Cadherin/CDH11 is at 120KD.

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Figure 2. IHC analysis of OB Cadherin/CDH11 using anti-OB Cadherin/CDH11 antibody (A04419-1).
OB Cadherin/CDH11 was detected in paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-OB Cadherin/CDH11 Antibody (A04419-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of OB Cadherin/CDH11 using anti-OB Cadherin/CDH11 antibody (A04419-1).
OB Cadherin/CDH11 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-OB Cadherin/CDH11 Antibody (A04419-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 4. Flow Cytometry analysis of PC-3 cells using anti-OB Cadherin/CDH11 antibody (A04419-1).
Overlay histogram showing PC-3 cells stained with A04419-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OB Cadherin/CDH11 Antibody (A04419-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For CDH11 (Source: Uniprot.org, NCBI)

Alternative Names

CAD11OSF-4; cadherin 11, type 2, OB-cadherin (osteoblast); Cadherin11; Cadherin-11; CDH11; CDHOB; OB; OB-Cadherin; Osteoblast cadherin CDH11 CAD11, CDHOB, ESWS, OB, OSF-4 cadherin 11 cadherin-11|cadherin 11, type 2, OB-cadherin (osteoblast)

For more info on CDH11, check out the CDH11 Infographic

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In this infographic, you will see the following information for CDH11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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