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Product Info Summary
| SKU: | A13456 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-OCC-1 Antibody
SKU/Catalog Number
A13456
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-OCC-1 Antibody (Catalog # A13456). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human.
Storage & Handling
OCC-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-OCC-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13456)
Host
Rabbit
Contents
OCC-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
OCC-1 antibody was raised against a 7 amino acid synthetic peptide near the carboxy terminus of human OCC-1. The immunogen is located within the last 50 amino acids of OCC-1.
Reactive Species
A13456 is reactive to C12ORF75 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
6.4 kDa
Background of C12ORF75
OCC-1 (Overexpressed in colon carcinoma-1) was initially identified as a novel human cDNA overexpressed in a colon carcinoma cell line. Elevated levels of OCC-1 mRNA were observed in three of eight colon carcinomas, suggesting that OCC-1 may be a hallmark of only a subset of colon carcinomas. It is highly expressed in skeletal muscle, kidney, placenta, and pancreas tissues, with low to no expression in heart, brain, lung and liver tissues. At least two different OCC-1 mRNA species are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13456 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
OCC-1 antibody can be used for detection of OCC-1 by Western blot at 2 - 4 μg/mL. In vitro transcription and translation of the OCC-1 cDNA yields polypeptides less than 9 kDa. Despite this, OCC-1 from cell lines is detected at ~28 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of OCC-1 in 293 cell lysate with OCC-1 antibody at (A) 2 μg/mL and (B) 4 μg/mL.
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Immunohistochemistry of OCC-1 in human spleen tissue with OCC-1 antibody at 2.5 μg/mL.
Specific Publications For Anti-OCC-1 Antibody (A13456)
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