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Product Info Summary
| SKU: | M01246 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-Occludin OCLN Rabbit Monoclonal Antibody
SKU/Catalog Number
M01246
BM4832 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Occludin OCLN Rabbit Monoclonal Antibody catalog # M01246. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Occludin OCLN Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01246)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IEH-15
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Occludin
Reactive Species
M01246 is reactive to OCLN in Human, Mouse, Rat
Observed Molecular Weight
59 kDa
Calculated molecular weight
59.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01246 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IP 1:20
FC 1:20
Positive Control
WB:human HepG2 whole cell, human 293T whole cell, human Caco-2 whole cell.
Validation Images & Assay Conditions
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Western blot analysis of OCLN using anti-OCLN antibody (M01246).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OCLN antigen affinity purified monoclonal antibody (M01246) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for OCLN at approximately 59 kDa. The expected band size for OCLN is at 59 kDa.
Specific Publications For Anti-Occludin OCLN Rabbit Monoclonal Antibody (M01246)
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Customer Q&As
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16 Customer Q&As for Anti-Occludin OCLN Rabbit Monoclonal Antibody
Question
I see that the anti-Occludin Rabbit Monoclonal antibody M01246 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-05-01
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon carcinoma using anti-Occludin Rabbit Monoclonal antibody M01246. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-13
Question
We want using your anti-Occludin Rabbit Monoclonal antibody for cell-cell junction organization studies. Has this antibody been tested with western blotting on hepg2 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
We appreciate your inquiry. This M01246 anti-Occludin Rabbit Monoclonal antibody is validated on hepg2 cell lysate. It is guaranteed to work for IP, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-21
Question
See below the WB image, lot number and protocol we used for colon carcinoma using anti-Occludin Rabbit Monoclonal antibody M01246. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-16
Question
I was wanting to use your anti-Occludin Rabbit Monoclonal antibody for IP for human colon carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human colon carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
It shows on the product datasheet, M01246 anti-Occludin Rabbit Monoclonal antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human colon carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-24
Question
Does anti-Occludin Rabbit Monoclonal antibody M01246 work for IP with colon carcinoma?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
According to the expression profile of colon carcinoma, OCLN is highly expressed in colon carcinoma. So, it is likely that anti-Occludin Rabbit Monoclonal antibody M01246 will work for IP with colon carcinoma.
Boster Scientific Support
Answered: 2019-09-18
Question
Can you help my question with product M01246, anti-Occludin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01246 anti-Occludin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-18
Question
Is there a BSA free version of anti-Occludin Rabbit Monoclonal antibody M01246 available?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Occludin Rabbit Monoclonal antibody M01246 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-21
Question
Does M01246 anti-Occludin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
As indicated on the product datasheet, M01246 anti-Occludin Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-14
Question
We have observed staining in human embryonic kidney. What should we do? Is anti-Occludin Rabbit Monoclonal antibody supposed to stain embryonic kidney positively?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
According to literature embryonic kidney does express OCLN. According to Uniprot.org, OCLN is expressed in thyroid gland, colon carcinoma, liver, brain lung, embryonic kidney, among other tissues. Regarding which tissues have OCLN expression, here are a few articles citing expression in various tissues:
Brain, and Lung, Pubmed ID: 15489334
Colon carcinoma, Pubmed ID: 8601611
Embryonic kidney, Pubmed ID: 17525332
Liver, Pubmed ID: 9175707, 18318008, 20463075, 24275569
Boster Scientific Support
Answered: 2019-06-11
Question
We ordered your anti-Occludin Rabbit Monoclonal antibody for WB on embryonic kidney in a previous experiment. I am using mouse, and We want to use the antibody for IP next. We are interested in examining embryonic kidney as well as thyroid gland in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2019-03-25
Answer
I took a look at the website and datasheets of our anti-Occludin Rabbit Monoclonal antibody and I see that M01246 has been tested on mouse in both WB and IP. Thus M01246 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-03-25
Question
Is a blocking peptide available for product anti-Occludin Rabbit Monoclonal antibody (M01246)?
Verified Customer
Verified customer
Asked: 2018-08-07
Answer
We do provide the blocking peptide for product anti-Occludin Rabbit Monoclonal antibody (M01246). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-08-07
Question
I was wanting to use to test anti-Occludin Rabbit Monoclonal antibody M01246 on human colon carcinoma for research purposes, then I may be interested in using anti-Occludin Rabbit Monoclonal antibody M01246 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-03-14
Answer
The products we sell, including anti-Occludin Rabbit Monoclonal antibody M01246, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-14
Question
Is this M01246 anti-Occludin Rabbit Monoclonal antibody reactive to the isotypes of OCLN?
Verified Customer
Verified customer
Asked: 2018-02-21
Answer
The immunogen of M01246 anti-Occludin Rabbit Monoclonal antibody is A synthesized peptide derived from human Occludin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-02-21
Question
My team were satisfied with the WB result of your anti-Occludin Rabbit Monoclonal antibody. However we have observed positive staining in colon carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is OCLN supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-08-30
Answer
From what I have seen in literature, colon carcinoma does express OCLN. Generally OCLN expresses in cell membrane. Regarding which tissues have OCLN expression, here are a few articles citing expression in various tissues:
Brain, and Lung, Pubmed ID: 15489334
Colon carcinoma, Pubmed ID: 8601611
Embryonic kidney, Pubmed ID: 17525332
Liver, Pubmed ID: 9175707, 18318008, 20463075, 24275569
Boster Scientific Support
Answered: 2017-08-30
Question
We are currently using anti-Occludin Rabbit Monoclonal antibody M01246 for mouse tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
O. Jones
Verified customer
Asked: 2017-08-08
Answer
The anti-Occludin Rabbit Monoclonal antibody (M01246) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-08
