Anti-Olfactory Marker Protein/OMP Antibody Picoband™

Product Name

Anti-Olfactory Marker Protein/OMP Antibody Picoband™

View all Olfactory Marker Protein Antibodies

SKU/Catalog Number

A01781

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Olfactory Marker Protein/OMP Antibody Picoband™ catalog # A01781. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Olfactory Marker Protein/OMP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01781)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human OMP recombinant protein (Position: R54-K137). Human OMP shares 91.7% amino acid (aa) sequence identity with both mouse and rat OMP.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A01781 is reactive to OMP in Human, Mouse, Rat

Applications

A01781 is guaranteed for WB Boster Guarantee

Background of Olfactory Marker Protein

Olfactory marker protein is uniquely associated with the mature olfactory receptor neurons in many vertebrate species from fish to man. The OMP gene structure and protein sequence are highly conserved between mouse, rat and human. Results of the mouse knockout studies show that OMP-null mice are compromised in their ability to respond to odor stimuli, and that OMP represents a novel modulatory component of the odor detection/signal transduction cascade.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of OMP using anti-OMP antibody (A01781).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: 22RV1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OMP antigen affinity purified polyclonal antibody (Catalog # A01781) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OMP at approximately 19KD. The expected band size for OMP is at 19KD.

Gene/Protein Information For OMP (Source: Uniprot.Org, NCBI)

Alternative Names

olfactory marker protein; Olfactory neuronal-specific protein OMP olfactory marker protein olfactory marker protein|olfactory neuronal-specific protein

For more info on OMP, check out the OMP Infographic

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In this infographic you will see the following information for OMP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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