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Product Info Summary
| SKU: | PB9732 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Oncostatin M/OSM Antibody Picoband®
SKU/Catalog Number
PB9732
PB0772 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Oncostatin M/OSM Antibody Picoband® catalog # PB9732. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Oncostatin M/OSM Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9732)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Oncostatin M recombinant protein (Position: A26-R220). Human Oncostatin M shares 47.1% and 47.6% amino acid (aa) sequence identity with mouse and rat Oncostatin M, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9732 is reactive to OSM in Human
Observed Molecular Weight
40 kDa
Calculated molecular weight
28.5 kDa
Background of OSM
OSM (ONCOSTATIN M) is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. OSM is mapped on 22q12.2. It has the ability to inhibit the growth of human A375 melanoma cells but not normal human fibroblasts. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM also has the ability to inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells, a function shared by LIF, CSF3, and IL6. The direction of gene transcription was telomeric to centromeric, with the OSM gene located upstream of the LIF gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9732 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: A549 Whole Cell, HUT Whole Cell, JURKAT Whole Cell, SW620 Whole Cell, MCF-7 Whole Cell
IHC: human intestinal cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Oncostatin M using anti-Oncostatin M antibody (PB9732).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: A549 Whole Cell Lysate,
Lane 2: HUT Whole Cell Lysate,
Lane 3: JURKAT Whole Cell Lysate,
Lane 4: SW620 Whole Cell Lysate,
Lane 5: MCF-7 Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Oncostatin M antigen affinity purified polyclonal antibody (Catalog # PB9732) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Oncostatin M at approximately 40 kDa. The expected band size for Oncostatin M is at 28 kDa.
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IHC analysis of Oncostatin M using anti-Oncostatin M antibody (PB9732).
Oncostatin M was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Oncostatin M Antibody (PB9732) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Oncostatin M/OSM Antibody Picoband® (PB9732)
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1 Customer Q&As for Anti-Oncostatin M/OSM Antibody Picoband®
Question
We are currently using anti-Oncostatin M/OSM antibody PB9732 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-07
Answer
The anti-Oncostatin M/OSM antibody (PB9732) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-07
