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Product Info Summary
| SKU: | A09717-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Monkey |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-OPTC Antibody Picoband®
SKU/Catalog Number
A09717-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-OPTC Antibody Picoband® catalog # A09717-1. Tested in ELISA, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-OPTC Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09717-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human OPTC recombinant protein (Position: E32-Q250).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09717-1 is reactive to OPTC in Human, Monkey
Observed Molecular Weight
37 kDa
Calculated molecular weight
37.3 kDa
Background of OPTC
Opticin is a protein that in humans is encoded by the OPTC gene. Opticin belongs to class III of the small leucine-rich repeat protein (SLRP) family. Members of this family are typically associated with the extracellular matrix. Opticin is present in significant quantities in the vitreous of the eye and also localizes to the cornea, iris, ciliary body, optic nerve, choroid, retina, and fetal liver. Opticin may noncovalently bind collagen fibrils and regulate fibril morphology, spacing, and organization. The opticin gene is mapped to a region of chromosome 1 that is associated with the inherited eye diseases age-related macular degeneration (AMD) and posterior column ataxia with retinosa pigmentosa (AXPC1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09717-1 is guaranteed for ELISA, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Human, Monkey |
| ELISA | 0.1-0.5 μg/ml | - |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of OPTC using anti-OPTC antibody (A09717-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human ARPE-19 whole cell lysates,
Lane 2: monkey RF/6A whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPTC antigen affinity purified polyclonal antibody (Catalog # A09717-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPTC at approximately 37 kDa. The expected band size for OPTC is at 37,45 kDa.
Specific Publications For Anti-OPTC Antibody Picoband® (A09717-1)
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