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Product Info Summary
| SKU: | A00909-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ORAI1 Antibody
SKU/Catalog Number
A00909-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ORAI1 Antibody (Catalog # A00909-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
ORAI1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ORAI1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00909-1)
Host
Rabbit
Contents
ORAI1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ORAI1 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human ORAI1. The immunogen is located within the first 50 amino acids of ORAI1.
Cross-reactivity
This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.
Reactive Species
A00909-1 is reactive to ORAI1 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
32.7 kDa
Background of ORAI1
Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00909-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 μg/mL in the (A) absence or (B) presence of blocking peptide.
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Immunohistochemistry of ORAI1 in human ovary tissue with ORAI1 antibody at 2.5 μg/ml.
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Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 μg/mL.
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Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 μg/mL.
Specific Publications For Anti-ORAI1 Antibody (A00909-1)
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