Product Info Summary
SKU: | M00909 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ORAI1 Monoclonal Antibody [3F6H5]
SKU/Catalog Number
M00909
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ORAI1 Monoclonal Antibody [3F6H5] (Catalog # M00909). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ORAI1 monoclonal antibody can be stored at -20°C, stable for one year.
Cite This Product
Anti-ORAI1 Monoclonal Antibody [3F6H5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00909)
Host
Mouse
Contents
ORAI1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
Clone: 3F6H5
Isotype
IgG1
Immunogen
Mouse monoclonal ORAI1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ORAI1 antibody is predicted to have no cross reactivity to other members in the ORAI family.
Reactive Species
M00909 is reactive to ORAI1 in Human, Mouse, Rat
Applications
M00909 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
32.668kDa
Background of ORAI1
Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one fo rm of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 5 μg/mL.
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Immunofluorescence of ORAI1 in human spleen tissue with ORAI1 antibody at 20 μg/ml.
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Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 2.5 μg/mL.
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Immunofluorescence of ORAI1 in human spleen tissue with ORAI1 antibody at 20 μg/ml.
Red: ORAI1 Antibody [3F6H5]
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For ORAI1 (Source: Uniprot.org, NCBI)
Gene Name
ORAI1
Full Name
Calcium release-activated calcium channel protein 1
Weight
32.668kDa
Superfamily
Orai family
Alternative Names
CRACM1; CRACM1ORAT1; FLJ14466; ORAI calcium release-activated calcium modulator 1; Orai1; ORAT1; Protein orai-1; TMEM142A; TMEM142Acalcium release-activated calcium modulator 1; Transmembrane protein 142Acalcium release-activated calcium channel protein 1 ORAI1 CRACM1, IMD9, ORAT1, TAM2, TMEM142A ORAI calcium release-activated calcium modulator 1 calcium release-activated calcium channel protein 1|calcium release-activated calcium modulator 1|protein orai-1|transmembrane protein 142A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ORAI1, check out the ORAI1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ORAI1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ORAI1 Monoclonal Antibody [3F6H5] (M00909)
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No publications found for M00909
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