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- Table of Contents
ORAI1 antibody (M00909) performs well in ELISA, IF, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence of ORAI1 in human spleen tissue with ORAI1 antibody at 20 μg/ml.
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Anti-ORAI1 Monoclonal Antibody [3F6H5]
ORAI1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | ELISA, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-ORAI1 Monoclonal Antibody [3F6H5]
View all ORAI1 Antibodies
View all ORAI1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
ORAI1 monoclonal antibody can be stored at 4°C, stable for one year.
Host
Mouse
Contents
ORAI1 Monoclonal Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Monoclonal
Clone Number
Clone: 3F6H5
Isotype
IgG1
Immunogen
Mouse monoclonal ORAI1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.
Cross-Reactivity
ORAI1 antibody is predicted to have no cross reactivity to other members in the ORAI family.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
32.7 kDa
Background of ORAI1
Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one fo rm of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.
Cite This Product
Anti-ORAI1 Monoclonal Antibody [3F6H5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00909)
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Product Promise
We promise Anti-ORAI1 Monoclonal Antibody [3F6H5] performs excellently in relevant applications for ELISA, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00909
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026