Anti-ORAI1 Monoclonal Antibody [3F6H5]

Product Name

Anti-ORAI1 Monoclonal Antibody [3F6H5]

View all ORAI1 Antibodies

SKU/Catalog Number

M00909

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-ORAI1 Monoclonal Antibody [3F6H5] (Catalog # M00909). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ORAI1 monoclonal antibody can be stored at -20°C, stable for one year.

Cite This Product

Anti-ORAI1 Monoclonal Antibody [3F6H5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00909)

Host

Mouse

Contents

ORAI1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

Clone: 3F6H5

Isotype

IgG1

Immunogen

Mouse monoclonal ORAI1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.

Cross-reactivity

ORAI1 antibody is predicted to have no cross reactivity to other members in the ORAI family.

Reactive Species

M00909 is reactive to ORAI1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

32.7 kDa

Background of ORAI1

Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one fo rm of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00909 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ORAI1

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