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- Table of Contents
ORAI3 antibody (M09399) performs well in ELISA, IF, IHC-P, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence of ORAI3 in rat spleen tissue with ORAI3 antibody at 5 μg/mL.
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Anti-ORAI3 Monoclonal Antibody [2H2G9]
ORAI3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Mouse | ELISA, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
ORAI3 antibody can be used for detection of ORAI3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-ORAI3 Monoclonal Antibody [2H2G9]
View all ORAI3 Antibodies
View all ORAI3 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
ORAI3 monoclonal antibody can be stored at 4°C, stable for one year.
Host
Mouse
Contents
ORAI3 Monoclonal Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Monoclonal
Clone Number
Clone: 2H2G9
Isotype
IgG2a
Immunogen
A 19 amino acid synthetic peptide from near the carboxy terminus of human ORAI3.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
31.5 kDa
Background of ORAI3
Antigen stimulation of immune cells triggers Ca++ entry t hrough Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. Larger molecular weight bands are sometimes seen in SDS-PAGE; these may represent post-translationally modified ORAI 3.
Cite This Product
Anti-ORAI3 Monoclonal Antibody [2H2G9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09399)
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Product Promise
We promise Anti-ORAI3 Monoclonal Antibody [2H2G9] performs excellently in relevant applications for ELISA, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M09399
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026