Anti-ORAI3 Monoclonal Antibody [2H2G9]

Product Name

Anti-ORAI3 Monoclonal Antibody [2H2G9]

View all ORAI3 Antibodies

SKU/Catalog Number

M09399

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-ORAI3 Monoclonal Antibody [2H2G9] (Catalog # M09399). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Rat.

Storage & Handling

ORAI3 monoclonal antibody can be stored at -20°C, stable for one year.

Cite This Product

Anti-ORAI3 Monoclonal Antibody [2H2G9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09399)

Host

Mouse

Contents

ORAI3 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

Clone: 2H2G9

Isotype

IgG2a

Immunogen

A 19 amino acid synthetic peptide from near the carboxy terminus of human ORAI3.

Reactive Species

M09399 is reactive to ORAI3 in Human, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

31.5 kDa

Background of ORAI3

Antigen stimulation of immune cells triggers Ca++ entry t hrough Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. Larger molecular weight bands are sometimes seen in SDS-PAGE; these may represent post-translationally modified ORAI 3.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M09399 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ORAI3 antibody can be used for detection of ORAI3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ORAI3

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