Anti-OS9 Antibody Picoband™

Product Name

Anti-OS9 Antibody Picoband™

View all OS9 Antibodies

SKU/Catalog Number

A04158-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-OS9 Antibody Picoband™ catalog # A04158-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-OS9 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04158-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human OS9 recombinant protein (Position: Q56-E635).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04158-1 is reactive to OS9 in Human, Mouse, Rat

Applications

A04158-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

83 kDa

Calculated molecular weight

75.562kDa

Background of OS9

Protein OS-9 is a protein that in humans is encoded by the OS9 gene. This gene encodes a protein that is highly expressed in osteosarcomas. This protein binds to the hypoxia-inducible factor 1 (HIF-1), a key regulator of the hypoxic response and angiogenesis, and promotes the degradation of one of its subunits. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of OS9 using anti-OS9 antibody (A04158-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HL-60 whole cell lysates,
Lane 4: rat pancreas tissue lysates,
Lane 5: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OS9 antigen affinity purified polyclonal antibody (Catalog # A04158-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OS9 at approximately 83 kDa. The expected band size for OS9 is at 76 kDa.

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Figure 2. IF analysis of OS9 using anti-OS9 antibody (A04158-1).
OS9 was detected in an immunocytochemical section of Siha cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OS9 Antibody (A04158-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For OS9 (Source: Uniprot.org, NCBI)

Alternative Names

Amplified in osteosarcoma 9; endoplasmic reticulum lectin 2; ERLEC2; erlectin 2; OS-9; osteosarcoma amplified 9, endoplasmic reticulum associated protein; osteosarcoma amplified 9, endoplasmic reticulum lectin; protein OS-9 OS9 ERLEC2, OS-9 OS9 endoplasmic reticulum lectin protein OS-9|amplified in osteosarcoma 9|endoplasmic reticulum lectin 2|erlectin 2|osteosarcoma amplified 9, endoplasmic reticulum associated protein|osteosarcoma amplified 9, endoplasmic reticulum lectin

For more info on OS9, check out the OS9 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for OS9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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