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SLC51B antibody (A12006) performs well in Flow Cytometry, IHC and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of HEPA1-6 cells using anti-SLC51B antibody (A12006).
Overlay histogram showing HEPA1-6 cells stained with A12006 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC51B Antibody (A12006, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HEPA1-6 cells stained with A12006 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC51B Antibody (A12006, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-OSTbeta/SLC51B Antibody
SLC51B antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | Flow Cytometry, IHC |
Tested Applications
SLC51B antibody was positively detected by IHC in:
mouse kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SLC51B antibody was positively detected by FC in:
HEPA1-6 cell
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Mouse
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-OSTbeta/SLC51B Antibody
View all SLC51B Antibodies
View all SLC51B Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human OSTbeta/SLC51B.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Calculated Molecular Weight
14.7 kDa
Background of SLC51B
Enables bile acid transmembrane transporter activity and protein heterodimerization activity. Involved in several processes, including bile acid and bile salt transport; positive regulation of establishment of protein localization; and positive regulation of protein glycosylation. Located in basolateral plasma membrane. Part of protein-containing complex. Is expressed in liver and small intestine. Orthologous to human SLC51B (SLC51 subunit beta).
Cite This Product
Anti-OSTbeta/SLC51B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12006)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
92%
High Homology
Dog
69%
Low homology
Human
65%
Low homology
Monkey
65%
Low homology
Cow
58%
Low homology
Rabbit
54%
Low homology
Sheep
54%
Low homology
Zebrafish
23%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for SLC51B antibody
Flow Cytometry (FC) protocol for SLC51B antibody
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Product Promise
We promise Anti-OSTbeta/SLC51B Antibody performs excellently in relevant applications for Flow Cytometry, IHC. If it doesn't perform as promised, we will refund the product. Learn more
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A12006
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026