Anti-Osteopontin SPP1 Rabbit Monoclonal Antibody

Boster Bio Anti-Osteopontin SPP1 Rabbit Monoclonal Antibody catalog # M00634. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse. Cited in 2 publication(s).

Product Info Summary

SKU: M00634
Size: 100 μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: Flow Cytometry, IF, ICC, WB

Product Name

Anti-Osteopontin SPP1 Rabbit Monoclonal Antibody

See all Osteopontin/OPN products

SKU/Catalog Number

M00634

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Osteopontin SPP1 Rabbit Monoclonal Antibody catalog # M00634. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Osteopontin SPP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00634)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

CEO-19

Immunogen

A synthesized peptide derived from human Osteopontin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00634 is reactive to SPP1 in Human, Mouse

Applications

M00634 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200
Flow Cytometry 1:30

Validation Images & Assay Conditions

Gene/Protein Information For SPP1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P10451

Gene ID

6696

Gene Name

SPP1

Full Name

Osteopontin

Weight

35.423kDa

Superfamily

osteopontin family

Alternative Names

BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin SPP1 BNSP, BSPI, ETA-1, OPN secreted phosphoprotein 1 osteopontin|SPP1/CALPHA1 fusion|early T-lymphocyte activation 1|nephropontin|osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein|secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)|secreted phosphoprotein 1 variant 6|urinary stone protein|uropontin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SPP1, check out the SPP1 Infographic

SPP1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SPP1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00634 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Expression of NF-κB and osteopontin of synovial fluid of patients with knee osteoarthritis

Effects of Naringin on Proliferation and Osteogenic Differentiation of Human Periodontal Ligament Stem Cells In Vitro and In Vivo

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Find answers in Q&As, reviews.

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16 Customer Q&As for Anti-Osteopontin SPP1 Rabbit Monoclonal Antibody

Question

I have a question about product M00634, anti-Osteopontin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-05-04

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00634 anti-Osteopontin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-05-04

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-Osteopontin Rabbit Monoclonal antibody M00634. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-04-16

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-16

Question

We are currently using anti-Osteopontin Rabbit Monoclonal antibody M00634 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-16

Answer

The anti-Osteopontin Rabbit Monoclonal antibody (M00634) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-16

Question

Would M00634 anti-Osteopontin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

G. Walker

Verified customer

Asked: 2020-03-04

Answer

As indicated on the product datasheet, M00634 anti-Osteopontin Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-04

Question

you antibody using your anti-Osteopontin Rabbit Monoclonal antibody for signaling by pdgf studies. Has this antibody been tested with western blotting on hepg2 cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-02-11

Answer

I appreciate your inquiry. This M00634 anti-Osteopontin Rabbit Monoclonal antibody is tested on hepg2 cell lysate. It is guaranteed to work for IF, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-02-11

Question

Would anti-Osteopontin Rabbit Monoclonal antibody M00634 work for WB with kidney?

B. Baker

Verified customer

Asked: 2020-01-24

Answer

According to the expression profile of kidney, SPP1 is highly expressed in kidney. So, it is likely that anti-Osteopontin Rabbit Monoclonal antibody M00634 will work for WB with kidney.

Boster Scientific Support

Answered: 2020-01-24

Question

My colleagues were content with the WB result of your anti-Osteopontin Rabbit Monoclonal antibody. However we have observed positive staining in placenta secreted. using this antibody. Is that expected? Could you tell me where is SPP1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

Based on literature, placenta does express SPP1. Generally SPP1 expresses in secreted. Regarding which tissues have SPP1 expression, here are a few articles citing expression in various tissues:
Brain, and Kidney, Pubmed ID: 15489334
Kidney, Pubmed ID: 1575754
Kidney, and Subthalamic nucleus, Pubmed ID: 14702039
Liver, Pubmed ID: 7945249, 24275569
Milk, Pubmed ID: 2736258, 15869464
Pituitary, Pubmed ID: 16807684
Placenta, Pubmed ID: 16303743

Boster Scientific Support

Answered: 2019-05-24

Question

Please see the WB image, lot number and protocol we used for kidney using anti-Osteopontin Rabbit Monoclonal antibody M00634. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-10-12

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-10-12

Question

I see that the anti-Osteopontin Rabbit Monoclonal antibody M00634 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-08-22

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-08-22

Question

I was wanting to use your anti-Osteopontin Rabbit Monoclonal antibody for WB for human kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human kidney identification?

Verified Customer

Verified customer

Asked: 2018-08-14

Answer

It shows on the product datasheet, M00634 anti-Osteopontin Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-08-14

Question

Is this M00634 anti-Osteopontin Rabbit Monoclonal antibody reactive to the isotypes of SPP1?

Verified Customer

Verified customer

Asked: 2018-05-29

Answer

The immunogen of M00634 anti-Osteopontin Rabbit Monoclonal antibody is A synthesized peptide derived from human Osteopontin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-05-29

Question

We have observed staining in mouse kidney. Are there any suggestions? Is anti-Osteopontin Rabbit Monoclonal antibody supposed to stain kidney positively?

R. Parker

Verified customer

Asked: 2018-02-15

Answer

According to literature kidney does express SPP1. According to Uniprot.org, SPP1 is expressed in amniotic fluid, liver, brain, kidney subthalamic nucleus, placenta, brain kidney, kidney, milk, pituitary, among other tissues. Regarding which tissues have SPP1 expression, here are a few articles citing expression in various tissues:
Brain, and Kidney, Pubmed ID: 15489334
Kidney, Pubmed ID: 1575754
Kidney, and Subthalamic nucleus, Pubmed ID: 14702039
Liver, Pubmed ID: 7945249, 24275569
Milk, Pubmed ID: 2736258, 15869464
Pituitary, Pubmed ID: 16807684
Placenta, Pubmed ID: 16303743

Boster Scientific Support

Answered: 2018-02-15

Question

Is there a BSA free version of anti-Osteopontin Rabbit Monoclonal antibody M00634 available?

Verified Customer

Verified customer

Asked: 2017-07-28

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Osteopontin Rabbit Monoclonal antibody M00634 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-07-28

Question

I am interested in to test anti-Osteopontin Rabbit Monoclonal antibody M00634 on human kidney for research purposes, then I may be interested in using anti-Osteopontin Rabbit Monoclonal antibody M00634 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

G. Li

Verified customer

Asked: 2013-10-28

Answer

The products we sell, including anti-Osteopontin Rabbit Monoclonal antibody M00634, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-10-28

Question

Is a blocking peptide available for product anti-Osteopontin Rabbit Monoclonal antibody (M00634)?

R. Li

Verified customer

Asked: 2013-07-22

Answer

We do provide the blocking peptide for product anti-Osteopontin Rabbit Monoclonal antibody (M00634). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-07-22

Question

We ordered your anti-Osteopontin Rabbit Monoclonal antibody for IF on kidney subthalamic nucleus a few months ago. I am using human, and We intend to use the antibody for WB next. We want examining kidney subthalamic nucleus as well as milk in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

D. Wu

Verified customer

Asked: 2013-02-05

Answer

I looked at the website and datasheets of our anti-Osteopontin Rabbit Monoclonal antibody and it seems that M00634 has been tested on human in both IF and WB. Thus M00634 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2013-02-05

Size

Total: $299

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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M00634
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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