Anti-Osteopontin SPP1 Rabbit Monoclonal Antibody

Western blot analysis of Osteopontin SPP1 using anti-Osteopontin SPP1 antibody (M00634).
Electrophoresis was performed on a 10% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse small intestine tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates,
Lane 7: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Osteopontin SPP1 antigen affinity purified monoclonal antibody (M00634) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Osteopontin SPP1 at approximately 35 kDa. The expected band size for Osteopontin SPP1 is at 35 kDa.

IHC analysis of SPP1 using anti-SPP1 antibody (M00634).
SPP1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SPP1 Antibody (M00634) overnight:50 at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IF staining images of (a) BMP-2, (b) COL-1, (c) OCN, and (d) OPN in BMSCs (red: osteogenic proteins and genes; green: cytoskeleton; blue: nucleus).
Index in World Scientific under a CC BY license. DOI: 10.1142/S1793545824420021

DFSC regulated tooth eruption through EVs. (A) Experimental design and schedule for model construction and result assessment. (B) Methods of measuring tooth eruption distance. (C) Representative micro-CT images of detecting tooth eruption distance. (D) Analysis of tooth eruption distance based on micro-CT. (E) Representative H&E staining images of the first mandibular molar area. (F) Analysis of tooth eruption distance based on H&E staining. (G) Representative immunohistochemistry staining images of OPN expression in the first mandibular molar area. (H) Quantitative analysis of OPN expression in the first mandibular molar area. (I) Representative images of TRAP staining. (J) Quantitative analysis of TRAP-positive distance. Scale bar = 1 mm ns, not significant. ** p < 0. 01. n = 3.
Index in PubMed under a CC BY license. PMID: 39834384

Immunofluorescent analysis using the Antibody at 1:50 dilution.

Prostaglandin E1 Inhibited Diabetes-Induced Phenotypic Switching of Vascular Smooth Muscle Cells Through Activating Autophagy. -Cellular Physiology and Biochemistry

Immunofluorescent analysis using the Antibody at 1:150 dilution.