Skip to the beginning of the images gallery

SPP1 antibody (M00634) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 24 Publications

Datasheet MSDS Request COA

View All Images (7)

Immunofluorescent analysis using the Antibody at 1:150 dilution.

Bundle Save

Bundle & Save Program

Save up to 50% on Picoband® antibodies + earn 10% back on eligible partner reagents.

View Details

BUY MORE, SAVE MORE

This antibody qualifies!
2 = 20% off · 3 = 30% off · 4 = 40% off · 5+ = 50% off

View Details

Free Secondary Antibody

How to redeem? While adding to cart, choose a size with a FREE Secondary Antibody!

View Details

Anti-Osteopontin SPP1 Rabbit Monoclonal Antibody

SPP1 antibody

24 Publications

Reactive Species	Host	Application
Human, Mouse	Rabbit	Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

SPP1 antibody was positively detected by WB in:

human Hela whole cell, human HepG2 whole cell, human MCF-7 whole cell, mouse kidney tissue, mouse small intestine, mouse NIH/3T3 whole cell, mouse RAW264.7 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

SPP1 antibody was positively detected by IHC in:

Vascular Smooth Muscle Cell

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

ICC

Human

Tested

Recommend

Mouse

Tested

Recommend

Product Information

Product Name

Anti-Osteopontin SPP1 Rabbit Monoclonal Antibody
View all SPP1 Antibodies

Form

Liquid

Uniprot

P10451

Gene ID

6696

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

CEO-19

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Osteopontin

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

35 kDa

Calculated Molecular Weight

35.4 kDa

Cite This Product

Anti-Osteopontin SPP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00634)

Protocols

Western Blot protocol for SPP1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for SPP1 antibody

Review Protocol

Publications

Cordycepin protects against simulated microgravity–induced osteogenic impairment involving ferroptosis-related signaling

Published: 2026-08-16

Journal: BIOCHEMICAL PHARMACOLOGY

Application: WB,IF

Impact Factor: 6.50

Species: Mouse

PMID: 42603620

Bundle Save

Bundle & Save Program

BUY MORE, SAVE MORE

Free Secondary Antibody

Anti-Osteopontin SPP1 Rabbit Monoclonal Antibody

SPP1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Cordycepin protects against simulated microgravity–induced osteogenic impairment involving ferroptosis-related signaling

Human Amniotic Mesenchymal Stromal Cells Promote Bone Regeneration via Regulating Ameloblastoma-Derived-Bone Marrow Mesenchymal Cells Crosstalk and Autophagy in Ameloblastoma Microenvironment

PTPRE Exacerbates Phenotypic Transformation of Vascular Smooth Muscle Cells and Vasomotor Dysfunction in Salt-Sensitive Hypertension

Immunomodulatory mesoporous bioactive glass/interleukin-4 nanocomplex promotes bone regeneration

“Triboelectric immunotherapy” powered osteogenesis and osteoimmune reprogramming for graft-free repair of bone defects

Targeting astrocytic CLC2(CLCN2) restores myelin regeneration through inhibition of SPP1/CD44 signaling pathway in leukoencephalopathy

Extracellular vesicles derived from dental follicle stem cells regulate tooth eruption by inhibiting osteoclast differentiation

Modified multilayered porous titanium scaffolds with silicon-doped coating surface-loaded BMP-2 prepared by microarc oxidation for bone defect repair

A photo-triggered bioactive nanohydrogel loaded with niobium carbide for stem cell osteogenic differentiation

CircLMBR1 inhibits phenotypic transformation of hypoxia-induced pulmonary artery smooth muscle via the splicing factor PUF60

Sulfated Laminarin Polysaccharides Reduce the Adhesion of Nano-COM Crystals to Renal Epithelial Cells by Inhibiting Oxidative and Endoplasmic Reticulum Stress

Carboxymethylated Desmodium styracifolium polysaccharide reduces the risk of calcium oxalate kidney stone formation by inhibiting crystal adhesion and promoting crystal endocytosis

Corn Silk Polysaccharide Reduces the Risk of Kidney Stone Formation by Reducing Oxidative Stress and Inhibiting COM Crystal Adhesion and Aggregation

Involvement of Cyclooxygenase-2 in Establishing an Immunosuppressive Microenvironment in Tumorspheres Derived from TMZ-Resistant Glioblastoma Cell Lines and Primary Cultures

PGC-1α affects cochlear pericytes migration in noise-exposed mice

Antioxidant Activities and Cytotoxicity of the Regulated Calcium Oxalate Crystals on HK-2 Cells of Polysaccharides from Gracilaria lemaneiformis with Different Molecular Weights

Insulin Resistance Promotes the Formation of Aortic Dissection by Inducing the Phenotypic Switch of Vascular Smooth Muscle Cells

Human Umbilical Cord Mesenchymal Stem Cells Attenuate Abdominal Aortic Aneurysm Progression in Sprague-Dawley Rats: Implication of Vascular Smooth Muscle Cell Phenotypic Modulation

An “all-in-one” scaffold targeting macrophages to direct endogenous bone repair in situ

Peptide LL-37 coating on micro-structured titanium implants to facilitate bone formation in vivo via mesenchymal stem cell recruitment

Expression of Both Matrix Metalloproteinase-2 and Its Tissue Inhibitor-2 in Tunica Media of Radial Artery in Uremic Patients

Prophylactic effects of Orthosiphon stamineus Benth. extracts on experimental induction of calcium oxalate nephrolithiasis in rats

The effects of mifepristone on the expressions of osteopontin, interleukin-6 and leukemia inhibitory factor in the villi of early pregnant women

Tranilast Attenuates Chronic Cyclosporine Nephrotoxicity in Rats

Review

Have you used Anti-Osteopontin SPP1 Rabbit Monoclonal Antibody?

Product Promise

Troubleshooting

Boster Guarantee

Guaranteed product quality