Anti-OXCT1/SCOT Antibody Picoband™

Product Name

Anti-OXCT1/SCOT Antibody Picoband™

View all OXCT1 Antibodies

SKU/Catalog Number

A07229-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-OXCT1/SCOT Antibody Picoband™ catalog # A07229-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-OXCT1/SCOT Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07229-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human OXCT1/SCOT recombinant protein (Position: S35-A309).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07229-1 is reactive to OXCT1 in Human, Monkey, Mouse, Rat

Applications

A07229-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

56 kDa

Calculated molecular weight

56.158kDa

Background of OXCT1

3-oxoacid CoA-transferase 1 (OXCT1) is an enzyme that in humans is encoded by the OXCT1 gene. It is also known as succinyl-CoA-3-oxaloacid CoA transferase (SCOT). This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat, Monkey
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of OXCT1/SCOT using anti-OXCT1/SCOT antibody (A07229-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: monkey heart tissue lysates,
Lane 4: human placenta tissue lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: rat kidney tissue lysates,
Lane 8: mouse heart tissue lysates,
Lane 9: mouse kidney tissue lysates,
Lane 10: mouse ANA-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXCT1/SCOT antigen affinity purified polyclonal antibody (Catalog # A07229-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OXCT1/SCOT at approximately 56KD. The expected band size for OXCT1/SCOT is at 56KD.

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Figure 2. IF analysis of OXCT1/SCOT using anti-OXCT1/SCOT antibody (A07229-1).
OXCT1/SCOT was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-OXCT1/SCOT Antibody (A07229-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of U87 cells using anti-OXCT1/SCOT antibody (A07229-1).
Overlay histogram showing U87 cells stained with A07229-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OXCT1/SCOT Antibody (A07229-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For OXCT1 (Source: Uniprot.org, NCBI)

Alternative Names

3-oxoacid CoA transferase 1,3-oxoacid CoA transferase; EC 2.8.3; EC 2.8.3.5; SCOTmitochondrial OXCT1 OXCT, SCOT 3-oxoacid CoA-transferase 1 succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial|3-oxoacid CoA transferase|SCOT-s|epididymis secretory sperm binding protein|somatic-type succinyl-CoA:3-oxoacid CoA-transferase|succinyl CoA:3-oxoacid CoA transferase|succinyl-CoA:3-ketoacid-CoA transferase

For more info on OXCT1, check out the OXCT1 Infographic

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In this infographic, you will see the following information for OXCT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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