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- Table of Contents
OXSM antibody (A12866-1) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Flow Cytometry analysis of MCF-7 cells using anti-OXSM antibody (A12866-1).
Overlay histogram showing MCF-7 cells stained with A12866-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OXSM Antibody (A12866-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing MCF-7 cells stained with A12866-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OXSM Antibody (A12866-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-OXSM Antibody Picoband®
OXSM antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
OXSM antibody was positively detected by WB in:
human Hela whole cell, human HepG2 whole cell, rat heart tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
OXSM antibody was positively detected by IHC in:
human Colorectal adenocarcinoma tissue, human endometrial adenocarcinoma tissue, human esophageal squamous carcinoma tissue, human liver cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
OXSM antibody was positively detected by ICC/IF in:
PC-3 cell
OXSM antibody was positively detected by FC in:
MCF-7 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-OXSM Antibody Picoband®
View all OXSM Antibodies
View all OXSM Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human OXSM recombinant protein (Position: M1-E431).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
46 kDa
Calculated Molecular Weight
48.8 kDa
Background of OXSM
This gene encodes a beta-ketoacyl synthetase. The encoded enzyme is required for elongation of fatty acid chains in the mitochondria. Alternatively spliced transcript variants have been described.
Cite This Product
Anti-OXSM Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12866-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Dog
92%
High Homology
Rabbit
89%
Moderate Homology
Rat
85%
Moderate Homology
Mouse
85%
Moderate Homology
Cow
83%
Moderate Homology
Sheep
82%
Moderate Homology
Zebrafish
67%
Low homology
Drosophila
51%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for OXSM antibody
Immunohistochemistry (IHC) protocol for OXSM antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for OXSM antibody
Flow Cytometry (FC) protocol for OXSM antibody
Publications
Application:
Species:
Multi-omics reveals PM2.5-Associated neuronal disulfidptosis: SLC3A2-OXSM imbalance, immune dysregulation, and electrophysiological dysfunction
Published: 2026-05-07
Journal: FOOD AND CHEMICAL TOXICOLOGY
Application: WB
Impact Factor: 3.20
Species: Rat
PMID: 42103060
Identifying disulfidptosis-related biomarkers in epilepsy based on integrated bioinformatics and experimental analyses
Published: 2025-01-11
Journal: NEUROBIOLOGY OF DISEASE
Application: IF,WB
Impact Factor: 5.60
Species: Rat
PMID: 39805370
Review
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Product Promise
We promise Anti-OXSM Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A12866-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026