Anti-P cadherin/CDH3 Antibody
Rabbit IgG polyclonal antibody for Cadherin-3(CDH3) detection. Tested with WB, IHC-P, ICC in Human.
Anti-P cadherin/CDH3 Antibody Info At A Glance
|Applications:||IHC, ICC, WB|
|Product Name||Anti-P cadherin/CDH3 Antibody
See all P-Cadherin primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for P CADHERIN/CDH3 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. P CADHERIN/CDH3 information: Molecular Weight: 91418 MW; Subcellular Localization: Cell membrane; Single-pass type I membrane protein; Tissue Specificity: Expressed in some normal epithelial tissues and in some carcinoma cell lines.|
|Cite This Product||Anti-P cadherin/CDH3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1363)|
|Similar Products From
|Anti-P cadherin/CDH3 Antibody may replace the following items: sc 6644|sc 8979|sc 5297|sc 59636|sc 6642|sc 59633|sc 59637.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Applications||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human P cadherin(612-626aa QDTYDVHLSLSDHGN), different from the mouse and rat sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For CDH3 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||1001|
|Species Of This Entry||Human|
|Alternative Names||P-Cadherin|CAD3; cadherin 3, P-cadherin (placental); cadherin 3, type 1, P-cadherin (placental); Cadherin-3; CDH3; CDHP; CDHPcalcium-dependent adhesion protein, placental; HJMD; PCAD; PCadherin; P-Cadherin; PCADP-cadherin; Placental cadherin|
|Post Tranlational Modifications||Methylation; Demethylation|
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein.|
|Gene Location||16q22.1, on Chromasome 16, gene sequence: NC_000016.10 (68644914..68727573)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CDH3 (Source: Uniprot.org, NCBI)
|Protein Function||Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.|
|Tissue Specificity||Expressed in some normal epithelial tissues and in some carcinoma cell lines.|
|Related Diseases||Malignant Neoplasms; Neoplasms; Carcinoma; Hypotrichosis; Dystrophy; Age Related Macular Degeneration; Tissue Adhesions; Malignant Neoplasm Of Breast; Blind Vision; Mammary Neoplasms; Colorectal Cancer; Hereditary Corneal Dystrophy; Dysplasia; Cell Invasion; Colorectal Neoplasms; Adenocarcinoma; Carcinogenesis; Neoplasm Metastasis; Neoplasm Invasiveness; Malignant Squamous Cell Neoplasm|
|Related Pathways||Cell Adhesion; Translation; Methylation; Demethylation; Pathogenesis; Cell Motility; Cell Cycle; Localization; Hair Follicle Morphogenesis; Catagen; Cell Migration; Immune Response; Limb Development; Inflammatory Response; Anagen; Invasive Growth; Cell-cell Adhesion; Cell Proliferation; Melanocyte Differentiation|
|Background||Cadherins, such as CDH3, are integral membrane glycoproteins responsible for calcium-dependent cell-cell adhesion. Cadherin-3 is a protein that in humans is encoded by the CDH3 gene. This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy.|
|Scientific References||PMID: 2793940 by Shimoyama Y., et al. Molecular cloning of a human Ca2+-dependent cell-cell adhesion molecule homologous to mouse placental cadherin: its low expression in human placental tissues.|
PMID: 9815605 by Jarrard D.F., et al. P-cadherin is a basal cell-specific epithelial marker that is not expressed in prostate cancer.
Our Boster Quality Guarantee for Anti-P cadherin/CDH3 Antibody covers its use in the following applications.Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
CDH3 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDH3 Antibody (PA1363) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
CDH3 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-CDH3 Antibody (PA1363) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human T-47D whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH3 antigen affinity purified polyclonal antibody (Catalog # PA1363) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDH3 at approximately 120KD. The expected band size for CDH3 is at 91KD.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Order Product (PA1363)
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.See Details
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.