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Cdkn1a antibody (M00145-8) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of p21/CDKN1A using anti-p21/CDKN1A antibody (M00145-8).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse RAW264.7 whole cell lysates,
Lane 2: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p21/CDKN1A antigen affinity purified monoclonal antibody (M00145-8) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for p21/CDKN1A at approximately 18 kDa. The expected band size for p21/CDKN1A is at 18 kDa.
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse RAW264.7 whole cell lysates,
Lane 2: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p21/CDKN1A antigen affinity purified monoclonal antibody (M00145-8) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for p21/CDKN1A at approximately 18 kDa. The expected band size for p21/CDKN1A is at 18 kDa.
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Anti-p21/CDKN1A Antibody (Monoclonal, 32C91)
Cdkn1a antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
Cdkn1a antibody was positively detected by WB in:
mouse RAW264.7 whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
1:500-2000
Immunohistochemistry
1:50-200
Immunocytochemistry/Immunofluorescence
1:50-200
Flow Cytometry (Fixed)
1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
FC
Mouse
Tested
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Product Information
Product Name
Anti-p21/CDKN1A Antibody (Monoclonal, 32C91)
View all CDKN1A Antibodies
View all CDKN1A Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
32C91
Immunogen
Recombinant protein within mouse p21.
Calculated Molecular Weight
17.8 kDa
Background of Cdkn1a
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at the G1 pahse. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Alternative splicing results in multiple transcript variants.
Cite This Product
Anti-p21/CDKN1A Antibody (Monoclonal, 32C91) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00145-8)
Protocols
Western Blot protocol for Cdkn1a antibody
Review
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Product Promise
We promise Anti-p21/CDKN1A Antibody (Monoclonal, 32C91) performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00145-8
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026