Anti-p21 CDKN1A Rabbit Monoclonal Antibody

Product Name

Anti-p21 CDKN1A Rabbit Monoclonal Antibody

View all p21/CIP1/CDKN1A Antibodies

SKU/Catalog Number

M00145-2

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-p21 CDKN1A Rabbit Monoclonal Antibody catalog # M00145-2. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-p21 CDKN1A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00145-2)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

EAI-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human p21

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00145-2 is reactive to CDKN1A in Human, Mouse, Rat

Applications

M00145-2 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

95 kDa

Calculated molecular weight

18.119kDa

Background of p21/CIP1/CDKN1A

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

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Immunofluorescent analysis using the Antibody at 1:150 dilution.

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Western blot analysis of p21 in (1) MCF-7 cell lysate; (2) HeLa cell lysate. (3) LnCap cell lysate; (4) U87 MG cell lysate.

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Immunofluorescent analysis of MCF7 cells, using p21 Antibody.

Gene/Protein Information For CDKN1A (Source: Uniprot.org, NCBI)

Alternative Names

CAP20cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; CDKN1A; CDKN1melanoma differentiation associated protein 6; CIP1; CIP1WAF1CDK-interaction protein 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); MDA6; MDA-6; Melanoma differentiation-associated protein 6; p21; p21CIP1; p21Cip1/Waf1; PIC1; SDI1DNA synthesis inhibitor; wild-type p53-activated fragment 1 CDKN1A CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1 cyclin dependent kinase inhibitor 1A cyclin-dependent kinase inhibitor 1|CDK-interacting protein 1|CDK-interaction protein 1|DNA synthesis inhibitor|cyclin-dependent kinase inhibitor 1A (p21, Cip1)|melanoma differentiation associated protein 6|wild-type p53-activated fragment 1

For more info on CDKN1A, check out the CDKN1A Infographic

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In this infographic, you will see the following information for CDKN1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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