Anti-p21 WAF1 CDKN1A Monoclonal Antibody

Product Name

Anti-p21 WAF1 CDKN1A Monoclonal Antibody

View all p21/CIP1/CDKN1A Antibodies

SKU/Catalog Number

M00145

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-p21 WAF1 CDKN1A Monoclonal Antibody (Catalog # M00145). Tested in ELISA, IF, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-p21 WAF1 CDKN1A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00145)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: WA-1 IgG1 kappa

Isotype

IgG1 kappa

Immunogen

This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p21 fusion protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00145 is reactive to CDKN1A in Human

Applications

M00145 is guaranteed for ELISA, IF, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

18.119kDa

Background of p21/CIP1/CDKN1A

p21WAF1 is a tumor suppressor gene product critical to maintain proper regulatory control of cell division. p21WAF1 inhibits cyclin/cyclin-dependent kinase (cdk) complexes, causing cell cycle arrest by blocking cell division at the S phase. Various mutations of tumor suppressor genes have been associated with different types of cancers. In these cases, somatic gene therapy with wild-type versions of tumor suppressor genes have been contemplated as anti-cancer therapeutic approaches. p21WAF1 contains p53-binding sites in its promoter and expression of p21WAF1 is induced in WI38 fibroblasts with PMA. Anti-p21WAF1 antibody is useful for researches interested in studying tongue and gastrointestinal cancers, as well as, ubiquitin and cyclin binding proteins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:20,000
IHC: User optimized
IF Microscopy: 1:250
IP: 1:100
WB: 1:2,000 - 1:10,000

Validation Images & Assay Conditions

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Western blot analysis of p21WAF1 expression in HeLa whole cell lysates (lane 1). p21WAF1 at 21KD was detected using mouse anti-p21WAF1 protein A purified monoclonal antibody (Catalog # M00145) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).

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p21 was detected in human brain tissues using mouse anti-p21 protein A purified monoclonal antibody (Catalog # M00145) at 1:250. Staining: p21 as precipitated blue with Cy5, YFP as precipitated green with Cy2, and Sox2 as precipitated red with Cy3. The immunohistochemical section was developed using SABC method (Catalog # SA1021).

Gene/Protein Information For CDKN1A (Source: Uniprot.org, NCBI)

Alternative Names

CAP20cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; CDKN1A; CDKN1melanoma differentiation associated protein 6; CIP1; CIP1WAF1CDK-interaction protein 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); MDA6; MDA-6; Melanoma differentiation-associated protein 6; p21; p21CIP1; p21Cip1/Waf1; PIC1; SDI1DNA synthesis inhibitor; wild-type p53-activated fragment 1 CDKN1A CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1 cyclin dependent kinase inhibitor 1A cyclin-dependent kinase inhibitor 1|CDK-interacting protein 1|CDK-interaction protein 1|DNA synthesis inhibitor|cyclin-dependent kinase inhibitor 1A (p21, Cip1)|melanoma differentiation associated protein 6|wild-type p53-activated fragment 1

For more info on CDKN1A, check out the CDKN1A Infographic

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In this infographic, you will see the following information for CDKN1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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