Anti-p23/PTGES3 Antibody Picoband™ (monoclonal, 9D3D1)

Product Name

Anti-p23/PTGES3 Antibody Picoband™ (monoclonal, 9D3D1)

View all p23/PTGES3 Antibodies

SKU/Catalog Number

M04136-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-p23/PTGES3 Antibody Picoband™ (monoclonal, 9D3D1) catalog # M04136-3. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-p23/PTGES3 Antibody Picoband™ (monoclonal, 9D3D1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04136-3)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

9D3D1

Isotype

Mouse IgG2b

Immunogen

E.coli-derived human p23/PTGES3 recombinant protein (Position: M1-K79).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M04136-3 is reactive to PTGES3 in Human, Mouse, Rat

Applications

M04136-3 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

23 kDa

Calculated molecular weight

18.697kDa

Background of p23/PTGES3

Prostaglandin E synthase 3 (cytosolic)is anenzymethat in humans is encoded by the PTGES3gene. It is mapped to 12q13.3; 12. This gene encodes an enzyme that converts prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). This protein functions as a co-chaperone with heat shock protein 90 (HSP90), localizing to response elements in DNA and disrupting transcriptional activation complexes. Alternative splicing results in multiple transcript variants. There are multiple pseudogenes of this gene on several different chromosomes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of p23/PTGES3 using anti-p23/PTGES3 antibody (M04136-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human COLO-320 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-p23/PTGES3 antigen affinity purified monoclonal antibody (Catalog # M04136-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for p23/PTGES3 at approximately 23 kDa. The expected band size for p23/PTGES3 is at 23 kDa.

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Figure 2. IF analysis of p23/PTGES3 using anti-p23/PTGES3 antibody (M04136-3).
p23/PTGES3 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-p23/PTGES3 Antibody (M04136-3) overnight at 4°C. DyLight®594 Conjugated Goat Anti-mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of PC-3 cells using anti-p23/PTGES3 antibody (M04136-3).
Overlay histogram showing PC-3 cells stained with M04136-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-p23/PTGES3 Antibody (M04136-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PTGES3 (Source: Uniprot.org, NCBI)

Alternative Names

CPGES; cPGESp23; Cytosolic prostaglandin E2 synthase; Hsp90 co-chaperone; p23; P23cytosolic prostaglandin E synthase; Progesterone receptor complex p23; prostaglandin E synthase 3 (cytosolic); prostaglandin E synthase 3; PTGES3; TEBP; TEBPEC 5.3.99.3; Telomerase-binding protein p23; unactive progesterone receptor, 23 kD PTGES3 P23, TEBP, cPGES prostaglandin E synthase 3 prostaglandin E synthase 3|Hsp90 co-chaperone|cytosolic prostaglandin E synthase|cytosolic prostaglandin E2 synthase|progesterone receptor complex p23|prostaglandin E synthase 3 (cytosolic)|telomerase-binding protein p23|unactive progesterone receptor, 23 kD

For more info on PTGES3, check out the PTGES3 Infographic

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In this infographic, you will see the following information for PTGES3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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