|Validated Species:||Human, Rat|
Data & Images
|Product Name||Anti-P27 KIP 1 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Cyclin-dependent kinase inhibitor 1B(CDKN1B) detection. Tested with WB in Humnan;Rat.|
|Cite This Product||Anti-P27 KIP 1 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9070)|
|Replacement Item||This antibody may replace the following items: sc-12939-R|sc-130603|sc-16324|sc-16324-R|sc-1641|sc-393305|sc-393380|sc-527|sc-528|sc-53871|sc-53906|sc-56338|sc-56454|sc-71813|sc-776 from Santa Cruz Biotechnology.|
|Validated Species||Human, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human P27 KIP 1 recombinant protein (Position: S10-T198). Human P27 KIP 1 shares 87% amino acid (aa) sequence identity with mouse P27 KIP 1.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Cyclin-dependent kinase inhibitor 1B|
|Molecular Weight||22073 MW|
|Protein Function||Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1- CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. .|
|Tissue Specificity||Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.|
|Sequence Similarities||Belongs to the CDI family.|
|Subcellular Localization||Nucleus. Cytoplasm. Endosome . Nuclear and cytoplasmic in quiescent cells. AKT- or RSK- mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen- activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity). .|
|Alternative Names||Cyclin-dependent kinase inhibitor 1B;Cyclin-dependent kinase inhibitor p27;p27Kip1;CDKN1B;KIP1;|
|Research Areas|||cell biology|cell cycle|cell cycle inhibitors|cip / kip| epigenetics and nuclear signaling|cip/kip| cancer||
Background for Cyclin-dependent kinase inhibitor 1B
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-P27 KIP 1 Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Rat, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-P27 KIP 1 Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti-p27 KIP 1(PB9070) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 27KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,