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- Table of Contents
CDKN1B antibody (PB9070) performs well in Flow Cytometry, IF, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
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Flow Cytometry analysis of SiHa cells using anti-p27 KIP 1/CDKN1B antibody (PB9070).
Overlay histogram showing SiHa cells stained with PB9070 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-p27 KIP 1/CDKN1B Antibody (PB9070, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing SiHa cells stained with PB9070 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-p27 KIP 1/CDKN1B Antibody (PB9070, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-p27 KIP 1/CDKN1B Antibody Picoband®
CDKN1B antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IF, ICC, WB |
Tested Applications
CDKN1B antibody was positively detected by WB in:
human Hela whole cell, human MCF-7 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CDKN1B antibody was positively detected by ICC/IF in:
U20S cell
CDKN1B antibody was positively detected by FC in:
SiHa cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
Human
Tested
Monkey
Predicted
Dog
Predicted
Sheep
Predicted
Bovine
Predicted
Product Information
Product Name
Anti-p27 KIP 1/CDKN1B Antibody Picoband®
View all CDKN1B Antibodies
View all CDKN1B Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human P27 KIP 1 recombinant protein (Position: S10-T198). Human P27 KIP 1 shares 87% amino acid (aa) sequence identity with mouse P27 KIP 1.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
27 kDa
Calculated Molecular Weight
22.1 kDa
Background of CDKN1B
Cyclin-dependent kinase inhibitor 1B (p27KIP1), also known as KIP1 or P27, is an enzyme inhibitor that in humans is encoded by the CDKN1B gene. It encodes a protein which belongs to the Cip/Kipfamily of cyclin dependent kinase (Cdk) inhibitor proteins. It is mapped to 12p13.1. p27KIP1 can inhibit both CDK activation and the kinase activity of assembled and activated cyclin-CDK. The function of p27KIP1 is associated with an aggressive phenotype in human breast cancer. Downregulation of p27KIP1 by CK2-alpha-prime is necessary for development of agonist- and stress-induced cardiac hypertrophy.
Cite This Product
Anti-p27 KIP 1/CDKN1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9070)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Dog
93%
High Homology
Sheep
91%
High Homology
Cow
90%
High Homology
Rabbit
89%
Moderate Homology
Mouse
86%
Moderate Homology
Rat
86%
Moderate Homology
Zebrafish
38%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CDKN1B antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CDKN1B antibody
Flow Cytometry (FC) protocol for CDKN1B antibody
Publications
Application:
Species:
Metallothionein 1 family profiling identifies MT1X as a tumor suppressor involved in the progression and metastastatic capacity of hepatocellular carcinoma
Published: 2018-06-06
Journal: MOLECULAR CARCINOGENESIS
Application: WB
Impact Factor: 3.85
Species: Human
PMID: 29873415
The oncoprotein HBXIP up-regulates Skp2 via activating transcription factor E2F1 to promote proliferation of breast cancer cells
Published: 2013-01-22
Journal: CANCER LETTERS
Application: WB
Impact Factor: 4.26
Species: Human
PMID: 23352642
ZFX regulates glioma cell proliferation and survival in vitro and in vivo
Published: 2013-01-16
Journal: JOURNAL OF NEURO-ONCOLOGY
Application: WB
Impact Factor: 3.12
Species: Human
PMID: 23322077
MicroRNA-155 inhibits proliferation and migration of human extravillous trophoblast derived HTR-8/SVneo cells via down-regulating cyclin D1
Published: 2012-08-01
Journal: PLACENTA
Application: WB
Impact Factor: 3.69
Species: Human
PMID: 22858023
Stat6 activity-related Th2 cytokine profile and tumor growth advantage of human colorectal cancer cells in vitro and in vivo
Published: 2011-11-13
Journal: CELLULAR SIGNALLING
Application: IHC
Impact Factor: 4.24
Species: Mouse
PMID: 22108090
Hepatitis B virus induces G1 phase arrest by regulating cell cycle genes in HepG2.2.15 cells
Published: 2011-05-15
Journal: Virology Journal
Application: WB
Impact Factor: 2.55
Species: Human
PMID: 21575146
Effects of Ad-p27mt gene transfer on the expression of Bax, Bcl-2, VEGF and MMP-9 in the transplanted liver tumors in nude mice
Published: 2010-11-10
Journal: Journal of Huazhong University of Science and Technology-Medical Sciences
Application: WB
Impact Factor: 0.31
Species: Human
PMID: 21063843
Dynamic changes of cell cycle elements in the ischemic brain after bone marrow stromal cells transplantation in rats
Published: 2009-09-30
Journal: NEUROSCIENCE LETTERS
Application: IHC
Impact Factor: 2.20
Species: Rat
PMID: 19799967
Chemically Modified Heparin Inhibits Mesangial Cell Proliferation Induced by High Glucose through Interfering with the Cell Cycle
Published: 2007-12-01
Journal: BIOLOGICAL & PHARMACEUTICAL BULLETIN
Application: WB
Impact Factor: 1.52
Species: Human
PMID: 18057711
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Product Promise
We promise Anti-p27 KIP 1/CDKN1B Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9070
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026