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- Table of Contents
CDKN1B antibody (M00173-4) performs well in ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Boster Kit Box
Designing a Western blot for CDKN1B? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the CDKN1B Western Blot GuideBundle Save
Anti-p27Kip1 CDKN1B Antibody (Monoclonal, 35C86)
CDKN1B antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | ICC, WB |
Recommend Dilution
Application
Dilution
Species
Immunocytochemistry
1μg/ml
Human, mouse, rat, -
Western blot
2-4μg/ml
Human, mouse, rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-p27Kip1 CDKN1B Antibody (Monoclonal, 35C86)
View all CDKN1B Antibodies
View all CDKN1B Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
35C86
Isotype
Mouse IgG1
Immunogen
recombinant rodent p27Kip1 protein.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Background of CDKN1B
P27 (kip1), also known as cyclin dependent kinase inhibitor 1B (CDKN1B), is a major target of AFX-like forkhead proteins. CDKN1B (p27) belongs to the Cip/Kip family and functions as an important cell cycle gatekeeper. Phosphorylation leads to the ubiquitination and degradation of CDKN1B. P27 (kip1) mapped to 12p13. An increase in p27 causes proliferating cells to exit from the cell cycle, and a decrease in p27 is necessary for quiescent cells to resume division. Abnormally low amounts of p27 are associated with pathological states of excessive cell proliferation, especially cancers. Overexpression of p27Kip1 lengthens the G1 phase in a mouse model that targets inducible gene expression to central nervous system progenitor cells.
Cite This Product
Anti-p27Kip1 CDKN1B Antibody (Monoclonal, 35C86) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00173-4)
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Product Promise
We promise Anti-p27Kip1 CDKN1B Antibody (Monoclonal, 35C86) performs excellently in relevant applications for ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00173-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 21, 2026