Anti-p28 ING5 Antibody

Product Name

Anti-p28 ING5 Antibody

View all Ing5 Antibodies

SKU/Catalog Number

A04974

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-p28 ING5 Antibody (Catalog # A04974). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-p28 ING5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04974)

Host

Goat

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 125-150 of Human p28 ING5 protein (Inhibitor of growth family, member 5).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04974 is reactive to Ing5 in Human

Applications

A04974 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

27.799kDa

Background of Ing5

p28 ING5 is a tumor suppressor protein similar to ING1 that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway.  Multiple alternatively spliced transcript variants have been observed. The accession number listed below is for variant (1) encodes the longest isoform.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:4,000 - 1:16,000
WB: 1:500 - 1:1,000

Validation Images & Assay Conditions

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Western blot analysis of p28 ING5 expression in purified recombinant human ING5 protein (lane 1). p28 ING5 at 36KD was detected using goat anti-p28 ING5 Antigen Affinity purified polyclonal antibody (Catalog # A04974) at 1:1500. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1003).

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Western blot analysis of p28 ING5 expression in HeLa cell nuclear extracts. This western blot shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4 on the same membrane (lane 2). A mock purification is shown in lane 1. p28 ING5 at 45KD was detected using goat anti-p28 ING5 Antigen Affinity purified polyclonal antibody (Catalog # A04974) at 1:500. Personal communication Jacques Cote.

Gene/Protein Information For Ing5 (Source: Uniprot.org, NCBI)

Alternative Names

inhibitor of growth family, member 5; inhibitor of growth protein 5; p28ING5FLJ23842

For more info on Ing5, check out the Ing5 Infographic

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In this infographic, you will see the following information for Ing5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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