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Product Info Summary
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Anti-P2RX7 Antibody Picoband™
Boster Bio Anti-P2RX7 Antibody Picoband™ catalog # A01208-2. Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-P2RX7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01208-2)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
E.coli-derived mouse P2RX7 recombinant protein (Position: N380-E585).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
A01208-2 is reactive to P2rx7 in Mouse
A01208-2 is guaranteed for ELISA, WB Boster Guarantee
Background of P2X7/P2RX7
P2X purinoceptor 7, also called P2X7, is a protein that in humans is encoded by the P2RX7 gene. The product of this gene belongs to the family of purnoceptors for ATP. P2RX7 was mapped to chromosome 12q24 by fluorescence in situ hybridization. It is a receptor for ATP that acts as a ligand-gated ion channel. This gene is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. It could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse
Direct ELISA, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of P2RX7 using anti-P2RX7 antibody (A01208-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: mouse Neuro-2a whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2RX7 antigen affinity purified polyclonal antibody (Catalog # A01208-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2RX7 at approximately 68KD. The expected band size for P2RX7 is at 68KD.
Gene/Protein Information For P2rx7 (Source: Uniprot.org, NCBI)
P2X purinoceptor 7
P2X receptor family
ATP receptor; MGC20089; P2RX7; P2X purinoceptor 7; P2X7; P2X7P2X7 receptor; P2X7R; P2Z receptor; purinergic receptor P2X, ligand-gated ion channel, 7; purinergic receptor P2X7 variant A; Purinergic receptor P2rx7|AI467586, P2X, P2X(7, P2X(7), P2X7R|purinergic receptor P2X, ligand-gated ion channel, 7|P2X purinoceptor 7|ATP receptor|P2X7 purinoceptor|P2X7 receptor|P2Z receptor*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on P2rx7, check out the P2rx7 Infographic
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In this infographic, you will see the following information for P2rx7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
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