Product Info Summary
SKU: | A03959-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-p60 CAF1 CHAF1B Antibody
SKU/Catalog Number
A03959-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-p60 CAF1 CHAF1B Antibody catalog # A03959-1. Tested in WB,ICC,IF,IP,IHC,Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p60 CAF1 CHAF1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03959-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human CEP55 around the phosphorylation site of S425.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03959-1 is reactive to CHAF1B in Human
Applications
A03959-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
61.493kDa
Background of CHAF1B
Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis.
Martinez-Garay I., Genomics 87:243-253(2006).
Shimokawa T., Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases.
Totoki Y., Submitted (APR-2005) to the EMBL/GenBank/DDBJ database.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
IP: 1:10-1:50
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of CHAF1B using Anti-p60 CAF1 CHAF1B Antibody (A03959-1).
ICC staining p60 CAF1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-p60 CAF1 CHAF1B Antibody A03959-1
Western blot analysis of p60 CAF1 on different cell lysates using anti-p60 CAF1 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For CHAF1B (Source: Uniprot.org, NCBI)
Gene Name
CHAF1B
Full Name
Chromatin assembly factor 1 subunit B
Weight
61.493kDa
Superfamily
WD repeat HIR1 family
Alternative Names
CAF1; CAF-1; CAF1ACAF-1 subunit B; CAF1P60CAF-I 60 kDa subunit; CAF-IP60; chromatin assembly factor 1 subunit B; chromatin assembly factor 1, subunit B (p60); Chromatin assembly factor I p60 subunit; human chromatin assembly factor-I p60 subunit, 10; MPHOSPH7CAF-I p60; MPP7M-phase phosphoprotein 7 CHAF1B CAF-1, CAF-IP60, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7 chromatin assembly factor 1 subunit B chromatin assembly factor 1 subunit B|CAF-1 subunit B|CAF-I 60 kDa subunit|CAF-I p60|M-phase phosphoprotein 7|chromatin assembly factor I p60 subunit|human chromatin assembly factor-I p60 subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHAF1B, check out the CHAF1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHAF1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p60 CAF1 CHAF1B Antibody (A03959-1)
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No publications found for A03959-1
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