Product Info Summary
| SKU: | A06811 |
|---|---|
| Size: | 100 µg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PACS-1 Antibody
SKU/Catalog Number
A06811
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-PACS-1 Antibody (Catalog# A06811). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-PACS-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06811)
Host
Rabbit
Contents
100 µg in 200 µl PBS containing 0.05% stabilizing protein and 0.05% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A partial length recombinant PACS-1 protein (amino acids 150-400)
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A06811 is reactive to PACS1 in Human
Calculated molecular weight
104.9 kDa
Background of PACS1
SmarcAL1 (SWI/SNF-related µatrix-associated actin-dependent regulator of chromatin a-like 1) is also known as HARP, HepA Related Protein, HHARP, SMARCA like Protein 1 and SWI/SNF Related. SmarcAL1 is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06811 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 1-3 µg/ml
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PACS-1. Anti-PACS-1 antibody was used at 1 &mu
Specific Publications For Anti-PACS-1 Antibody (A06811)
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Customer Q&As
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6 Customer Q&As for Anti-PACS-1 Antibody
Question
I am interested in to test anti-PACS-1 antibody A06811 on human liver for research purposes, then I may be interested in using anti-PACS-1 antibody A06811 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
The products we sell, including anti-PACS-1 antibody A06811, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-28
Question
Is a blocking peptide available for product anti-PACS-1 antibody (A06811)?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
We do provide the blocking peptide for product anti-PACS-1 antibody (A06811). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-01-03
Question
We are currently using anti-PACS-1 antibody A06811 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
T. Rodriguez
Verified customer
Asked: 2019-10-07
Answer
The anti-PACS-1 antibody (A06811) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-07
Question
Can you help my question with product A06811, anti-PACS-1 antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06811 anti-PACS-1 antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-16
Question
I was wanting to use your anti-PACS-1 antibody for WB for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
As indicated on the product datasheet, A06811 anti-PACS-1 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-14
Question
Will A06811 anti-PACS-1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-03-08
Answer
It shows on the product datasheet, A06811 anti-PACS-1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-03-08

