Anti-PAG1 Purified Monoclonal Antibody

Product Name

Anti-PAG1 Purified Monoclonal Antibody

View all PAG1 Antibodies

SKU/Catalog Number

M03711

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-PAG1 Purified Monoclonal Antibody (Catalog# M03711). Tested in IP, WB, IHC-P application(s). This antibody reacts with Rat, Cow, Human, Mouse.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-PAG1 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03711)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

PAG-C1

Isotype

Mouse IgG2b

Immunogen

C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH. The antibody PAG-C1 recognizes an epitope located in the intracellular C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M03711 is reactive to PAG1 in Cow, Human, Mouse, Rat

Applications

M03711 is guaranteed for IP, IHC-P, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

46.981kDa

Background of PAG1

PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (paraffin sections): Positive tissue: appendix (germinal center of lymphatic follicle), heat-mediated antigen retrieval in citrate buffer pH 6.1.
Immunoprecipitation: Positive control: RAJI human Burkitt lymphoma cell line.

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of PAG1 using Anti-PAG1 Purified Monoclonal Antibody (M03711).

Immunohistochemistry staining (paraffin sections) of PAG/Cbp in germinal center of lymphatic follicle and in dispersed T cells in appendix tissue by monoclonal antibody PAG-C1. Positive signal in T cells.
For more protocol information of IHC

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Figure 1. Western blotting validation for Anti-PAG1 Purified Monoclonal Antibody M03711

Immunoprecipitation of human PAG/Cbp from the lysate of RAJI human Burkitt lymphoma cell line.
Western blot was immunostained with anti-human PAG (MEM-255; cat. no. 11-347-C100).
Note: PAG/Cbp is a 46 kDa adaptor protein

Gene/Protein Information For PAG1 (Source: Uniprot.org, NCBI)

Alternative Names

CBP; CBPMGC138364; Csk-binding protein; PAG1; PAGFLJ37858; phosphoprotein associated with glycosphingolipid microdomains 1; phosphoprotein associated with glycosphingolipid-enriched microdomains 1; Transmembrane adapter protein PAG; Transmembrane phosphoprotein Cbp PAG1 CBP, PAG phosphoprotein membrane anchor with glycosphingolipid microdomains 1 phosphoprotein associated with glycosphingolipid-enriched microdomains 1|Csk-binding protein|phosphoprotein associated with glycosphingolipid microdomains 1|transmembrane adapter protein PAG|transmembrane adaptor protein PAG|transmembrane phosphoprotein Cbp

For more info on PAG1, check out the PAG1 Infographic

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In this infographic, you will see the following information for PAG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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