Anti-PAK2 Antibody

Product Name

Anti-PAK2 Antibody

View all PAK2 Antibodies

SKU/Catalog Number

A01419-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-PAK2 Antibody (Catalog # A01419-2). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

PAK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-PAK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01419-2)

Host

Rabbit

Contents

PAK2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-PAK2 antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human PAK2. The immunogen is located within amino acids 440-490 of PAK2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

TRB3 antibody is human, mouse and rat reactive. At least two isoforms of TRB3 are known to exist; this antibody will detect both isoforms.

Reactive Species

A01419-2 is reactive to PAK2 in Human, Mouse, Rat

Applications

A01419-2 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

58.043kDa

Background of PAK2

The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6, and are not activated by Cdc42/Rac binding. Of the six PAK proteins, only PAK2 is ubiquitously expressed and cleaved by caspase-3. This cleavage removes the amino-terminal regulatory domain and generates a constitutively active kinase fragment. Recent experiments have shown that following cleavage, the active fragment is myristoylated and directed to the plasma membrane and membrane ruffles where it promotes cell death via increased signaling through the c-Jun N-terminal kinase pathway, but without compromising mitochondrial integrity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 0.5-1 μg/mL; IHC: 10 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Click image to see more details

Western Blot Validation in Rat Bladder Tissue Lysate
Loading: 15 μg of lysates per lane.Antibodies: PAK2 A01419-2 (A: 0.5 μg/mL, B: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Click image to see more details

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: PAK2 A01419-2 (1 μg/mL), PAK2 (1 μg/mL) and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Click image to see more details

Western Blot Validation in Human, Mouse and Rat Cell Lines
Loading: 15 μg of lysates per lane. Antibodies: PAK2 A01419-2 (1 μg/mL per lane), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Click image to see more details

Western Blot Validation in Mouse Tissues
Loading: 15 μg of lysates per lane. Antibodies: PAK2 A01419-2 (1 μg/mL per lane)

Click image to see more details

Immunohistochemistry Validation of PAK2 in Mouse Spleen Tissue
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PAK2 antibody (A01419-2) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Click image to see more details

Immunofluorescence Validation of PAK2 in Mouse Spleen Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse spleen labeling PAK2 with A01419-2 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Gene/Protein Information For PAK2 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.11; gamma-PAK; p21 (CDKN1A)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 2; p21-activated kinase 2; p58; PAK2; PAK-2; PAK65; PAK65EC 2.7.11.1; PAKgamma; S6/H4 Kinase; serine/threonine-protein kinase PAK 2 PAK2 PAK65, PAKgamma p21 (RAC1) activated kinase 2 serine/threonine-protein kinase PAK 2|PAK-2|S6/H4 kinase|gamma-PAK|p21 (CDKN1A)-activated kinase 2|p21 protein (Cdc42/Rac)-activated kinase 2|p21-activated kinase 2|p58

For more info on PAK2, check out the PAK2 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PAK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the PAK2 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis