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Product Info Summary
| SKU: | M01419 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PAK2 Monoclonal Antibody
SKU/Catalog Number
M01419
BM5434 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PAK2 Monoclonal Antibody catalog # M01419. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAK2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01419)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADFE-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PAK2 The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD.
Reactive Species
M01419 is reactive to PAK2 in Human, Mouse, Rat
Observed Molecular Weight
61 kDa
Calculated molecular weight
58.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01419 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:80
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PAK2 expression in HeLa cell lysate.
Specific Publications For Anti-PAK2 Monoclonal Antibody (M01419)
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5 Customer Q&As for Anti-PAK2 Monoclonal Antibody
Question
We are currently using anti-PAK2 Monoclonal antibody M01419 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
The anti-PAK2 Monoclonal antibody (M01419) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-17
Question
What is the sample target on Western blot analysis in your product image for M01419?
Verified customer
Asked: 2019-12-09
Answer
Western blot analysis of the Anti-PAK2 Monoclonal Antibody (M01419) expression in HeLa cell lysate.
Boster Scientific Support
Answered: 2019-12-10
Question
We are interested in to test anti-PAK2 Monoclonal antibody M01419 on human cervix carcinoma for research purposes, then I may be interested in using anti-PAK2 Monoclonal antibody M01419 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Dhar
Verified customer
Asked: 2019-01-31
Answer
The products we sell, including anti-PAK2 Monoclonal antibody M01419, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-31
Question
Would anti-PAK2 Monoclonal antibody M01419 work on goat WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2017-07-12
Answer
Our lab technicians have not tested anti-PAK2 Monoclonal antibody M01419 on goat. You can run a BLAST between goat and the immunogen sequence of anti-PAK2 Monoclonal antibody M01419 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-12
Question
Is this M01419 anti-PAK2 Monoclonal antibody reactive to the isotypes of PAK2?
R. Parker
Verified customer
Asked: 2014-11-19
Answer
The immunogen of M01419 anti-PAK2 Monoclonal antibody is A synthesized peptide derived from human PAK2 The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-11-19
