Product Info Summary
SKU: | M01419 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PAK2 Monoclonal Antibody
SKU/Catalog Number
M01419
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PAK2 Monoclonal Antibody catalog # M01419. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAK2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01419)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADFE-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PAK2 The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M01419 is reactive to PAK2 in Human, Mouse, Rat
Applications
M01419 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
40 kDa
Calculated molecular weight
58.043kDa
Background of PAK2
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:500
ICC/IF 1:50-1:200
FC 1:80
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PAK2 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PAK2 (Source: Uniprot.org, NCBI)
Gene Name
PAK2
Full Name
Serine/threonine-protein kinase PAK 2
Weight
58.043kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; gamma-PAK; p21 (CDKN1A)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 2; p21-activated kinase 2; p58; PAK2; PAK-2; PAK65; PAK65EC 2.7.11.1; PAKgamma; S6/H4 Kinase; serine/threonine-protein kinase PAK 2 PAK2 PAK65, PAKgamma p21 (RAC1) activated kinase 2 serine/threonine-protein kinase PAK 2|PAK-2|S6/H4 kinase|gamma-PAK|p21 (CDKN1A)-activated kinase 2|p21 protein (Cdc42/Rac)-activated kinase 2|p21-activated kinase 2|p58
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAK2, check out the PAK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PAK2 Monoclonal Antibody (M01419)
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No publications found for M01419
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Recommended Resources
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-PAK2 Monoclonal Antibody
Question
We are currently using anti-PAK2 Monoclonal antibody M01419 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
The anti-PAK2 Monoclonal antibody (M01419) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-17
Question
What is the sample target on Western blot analysis in your product image for M01419?
Verified customer
Asked: 2019-12-09
Answer
Western blot analysis of the Anti-PAK2 Monoclonal Antibody (M01419) expression in HeLa cell lysate.
Boster Scientific Support
Answered: 2019-12-10
Question
We are interested in to test anti-PAK2 Monoclonal antibody M01419 on human cervix carcinoma for research purposes, then I may be interested in using anti-PAK2 Monoclonal antibody M01419 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Dhar
Verified customer
Asked: 2019-01-31
Answer
The products we sell, including anti-PAK2 Monoclonal antibody M01419, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-31
Question
Would anti-PAK2 Monoclonal antibody M01419 work on goat WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2017-07-12
Answer
Our lab technicians have not tested anti-PAK2 Monoclonal antibody M01419 on goat. You can run a BLAST between goat and the immunogen sequence of anti-PAK2 Monoclonal antibody M01419 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-12
Question
Is this M01419 anti-PAK2 Monoclonal antibody reactive to the isotypes of PAK2?
R. Parker
Verified customer
Asked: 2014-11-19
Answer
The immunogen of M01419 anti-PAK2 Monoclonal antibody is A synthesized peptide derived from human PAK2 The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-11-19