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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
|Application:||Flow Cytometry, IF, IHC, ICC, WB|
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Anti-PAK2 Monoclonal Antibody
Boster Bio Anti-PAK2 Monoclonal Antibody catalog # M01419. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAK2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01419)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human PAK2 The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M01419 is reactive to PAK2 in Human, Mouse, Rat
M01419 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
WB 1:500~1:2000 IHC 1:100~1:500 ICC/IF 1:50~1:200 FC 1:80
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PAK2 expression in HeLa cell lysate using anti-PAK2 antibody (M01419).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK2 antigen affinity purified polyclonal antibody (Catalog # M01419) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for PAK2.
Gene/Protein Information For PAK2 (Source: Uniprot.org, NCBI)
Serine/threonine-protein kinase PAK 2
protein kinase superfamily
EC 2.7.11; gamma-PAK; p21 (CDKN1A)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 2; p21-activated kinase 2; p58; PAK2; PAK-2; PAK65; PAK65EC 184.108.40.206; PAKgamma; S6/H4 Kinase; serine/threonine-protein kinase PAK 2 PAK2 PAK65, PAKgamma p21 (RAC1) activated kinase 2 serine/threonine-protein kinase PAK 2|PAK-2|S6/H4 kinase|gamma-PAK|p21 (CDKN1A)-activated kinase 2|p21 protein (Cdc42/Rac)-activated kinase 2|p21-activated kinase 2|p58*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on PAK2, check out the PAK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
No publications found for M01419
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