Click image to see more details
Product Info Summary
| SKU: | M04207 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-PAR6 Monoclonal Antibody
SKU/Catalog Number
M04207
BM5559 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PAR6 Monoclonal Antibody catalog # M04207. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAR6 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04207)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEDE-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PAR6 Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly.
Reactive Species
M04207 is reactive to PARD6A in Human, Mouse, Rat
Observed Molecular Weight
42 kDa
Calculated molecular weight
37.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04207 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:50
Positive Control
WB: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of PAR6 expression in Jurkat cell lysate.
Specific Publications For Anti-PAR6 Monoclonal Antibody (M04207)
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Customer Q&As
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7 Customer Q&As for Anti-PAR6 Monoclonal Antibody
Question
Does M04207 anti-PAR6 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
It shows on the product datasheet, M04207 anti-PAR6 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-13
Question
Is this M04207 anti-PAR6 Monoclonal antibody reactive to the isotypes of PARD6A?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
The immunogen of M04207 anti-PAR6 Monoclonal antibody is A synthesized peptide derived from human PAR6 Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-22
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right hemisphere of cerebellum using anti-PAR6 Monoclonal antibody M04207. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-22
Question
See attached the WB image, lot number and protocol we used for right hemisphere of cerebellum using anti-PAR6 Monoclonal antibody M04207. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-15
Question
We are currently using anti-PAR6 Monoclonal antibody M04207 for rat tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
N. Anderson
Verified customer
Asked: 2018-10-10
Answer
The anti-PAR6 Monoclonal antibody (M04207) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-10
Question
I see that the anti-PAR6 Monoclonal antibody M04207 works with WB, what is the protocol used to produce the result images on the product page?
K. Banerjee
Verified customer
Asked: 2018-02-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-02-16
Question
Is a blocking peptide available for product anti-PAR6 Monoclonal antibody (M04207)?
C. Parker
Verified customer
Asked: 2013-01-24
Answer
We do provide the blocking peptide for product anti-PAR6 Monoclonal antibody (M04207). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2013-01-24
