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Product Info Summary
| SKU: | A13680 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Paralemmin3 PALM3 Antibody
SKU/Catalog Number
A13680
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Paralemmin3 PALM3 Antibody (Catalog # A13680). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
Paralemmin3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Paralemmin3 PALM3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13680)
Host
Rabbit
Contents
Paralemmin3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Paralemmin3 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human Paralemmin3. The immunogen is located within amino acids 50 - 100 of Paralemmin3.
Cross-reactivity
At least two isoforms of Paralemmin3 are known to exist; this antibody will only detect the longer isoform
Reactive Species
A13680 is reactive to PALM3 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
71.7 kDa
Background of PALM3
Paralemmin3 is a recently identified protein that associates with the single immunoglobulin IL-1 receptor-related molecule (SIGIRR), a negative regulator of Toll-like receptor-interleukin 1 receptor signaling. Silencing of Paralemmin3 expression by RNAi inhibited the release of inflammatory cytokines in A549 cells after stimulation with lipopolysaccharide (LPS), suggesting Paralemmin3 may function as an adaptor in the LPS-TLR4 signaling pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13680 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Paralemmin3 antibody can be used for detection of Paralemmin3 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Paralemmin3 in MCF7 cell lysate with Paralemmin3 antibody at 1 μg/mL.
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Immunocytochemistry of Paralemmin 3 in MCF7 cells with Paralemmin 3 antibody at 5 μg/mL.
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Immunofluorescence of Paralemmin 3 in MCF7 cells with Paralemmin 3 antibody at 20 μg/mL.
Specific Publications For Anti-Paralemmin3 PALM3 Antibody (A13680)
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