Anti-PAX6 Monoclonal Antibody

Product Name

Anti-PAX6 Monoclonal Antibody

View all PAX6 Antibodies

SKU/Catalog Number

M00273

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-PAX6 Monoclonal Antibody (Catalog # M00273). Tested in ELISA, IF applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-PAX6 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00273)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 7H10.D3.D4.G5 IgG1 kappa

Isotype

IgG1 kappa

Immunogen

PAX6 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to human PAX6 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00273 is reactive to PAX6 in Human

Applications

M00273 is guaranteed for ELISA, IF Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

46.683kDa

Background of PAX6

Paired box protein Pax-6 also known as aniridia type II protein (AN2) or oculorhombin is a transcription factor with key functions in the development of the eye, nose, central nervous system and pancreas. Pax6 further seems to be required for the differentiation of pancreatic islet alpha cells. PAX6 has been shown to bind to a promoter element in the glucagon, insulin and somatostatin promoters and regulates the specification of the ventral neuron subtypes by establishing the correct progenitor domains. Isoform 5a appears to function as a molecular switch that specifies target genes. Anti-PAX6 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:75,000 - 1:150,000
IF Microscopy: 1:100
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

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PAX6 was detected in Immunofluorescence of Hela Cell, 20X using mouse anti-PAX6 Antigen Affinity purified monoclonal antibody (Catalog # M00273) at 1:100. Secondary antibody: goat anti-mouse secondary antibody at 1:1,000 for 45 min at RT. Localization: PAX6 is nuclear. Staining: Merged image of PAX6 as red fluorescent signal with DAPI (blue) nuclear counterstain. Images courtesy of Yang Xiang with Boston Children's Hospital.

Gene/Protein Information For PAX6 (Source: Uniprot.org, NCBI)

Alternative Names

keratitis); MGC17209; Oculorhombin; paired box 6; paired box protein Pax-6; Pax6 PAX6 AN, AN1, AN2, ASGD5, D11S812E, FVH1, MGDA, WAGR paired box 6 paired box protein Pax-6|Aniridia 1|Aniridia 2|aniridia type II protein|oculorhombin|paired box homeotic gene-6

For more info on PAX6, check out the PAX6 Infographic

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In this infographic, you will see the following information for PAX6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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