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Product Info Summary
| SKU: | M00943-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PAX8 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00943-2
BM4807 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PAX8 Rabbit Monoclonal Antibody catalog # M00943-2. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAX8 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00943-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ICE-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PAX8
Reactive Species
M00943-2 is reactive to PAX8 in Human
Observed Molecular Weight
48 kDa
Calculated molecular weight
48.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00943-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of PAX8 expression in SK-OV-3 cell lysate.
Specific Publications For Anti-PAX8 Rabbit Monoclonal Antibody (M00943-2)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-PAX8 Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-PAX8 Rabbit Monoclonal antibody (M00943-2)?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
We do provide the blocking peptide for product anti-PAX8 Rabbit Monoclonal antibody (M00943-2). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-04-08
Question
I was wanting to use to test anti-PAX8 Rabbit Monoclonal antibody M00943-2 on human right lobe of thyroid gland for research purposes, then I may be interested in using anti-PAX8 Rabbit Monoclonal antibody M00943-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
The products we sell, including anti-PAX8 Rabbit Monoclonal antibody M00943-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-03
Question
I was wanting to use your anti-PAX8 Rabbit Monoclonal antibody for IF for human right lobe of thyroid gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human right lobe of thyroid gland identification?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
It shows on the product datasheet, M00943-2 anti-PAX8 Rabbit Monoclonal antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human right lobe of thyroid gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-10
Question
We are currently using anti-PAX8 Rabbit Monoclonal antibody M00943-2 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
The anti-PAX8 Rabbit Monoclonal antibody (M00943-2) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-10
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right lobe of thyroid gland using anti-PAX8 Rabbit Monoclonal antibody M00943-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-27
Question
Do you have a BSA free version of anti-PAX8 Rabbit Monoclonal antibody M00943-2 available?
M. Dhar
Verified customer
Asked: 2019-03-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PAX8 Rabbit Monoclonal antibody M00943-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-03-13
Question
We bought anti-PAX8 Rabbit Monoclonal antibody for WB on cervix in the past. I am using human, and We intend to use the antibody for IF next. I was wanting to use examining cervix as well as right lobe of thyroid gland in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
I viewed the website and datasheets of our anti-PAX8 Rabbit Monoclonal antibody and it seems that M00943-2 has been tested on human in both WB and IF. Thus M00943-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-02-21
Question
We have been able to see staining in human cervix. Are there any suggestions? Is anti-PAX8 Rabbit Monoclonal antibody supposed to stain cervix positively?
Verified Customer
Verified customer
Asked: 2018-07-19
Answer
Based on literature cervix does express PAX8. Based on Uniprot.org, PAX8 is expressed in right lobe of thyroid gland, kidney, cervix, among other tissues. Regarding which tissues have PAX8 expression, here are a few articles citing expression in various tissues:
Cervix, Pubmed ID: 14702039
Kidney, Pubmed ID: 1337742, 8413205, 15489334
Boster Scientific Support
Answered: 2018-07-19
Question
Here is the WB image, lot number and protocol we used for right lobe of thyroid gland using anti-PAX8 Rabbit Monoclonal antibody M00943-2. Please let me know if you require anything else.
A. Parker
Verified customer
Asked: 2018-03-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-06
Question
My colleagues were content with the WB result of your anti-PAX8 Rabbit Monoclonal antibody. However we have seen positive staining in kidney nucleus. using this antibody. Is that expected? Could you tell me where is PAX8 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-10-17
Answer
From literature, kidney does express PAX8. Generally PAX8 expresses in nucleus. Regarding which tissues have PAX8 expression, here are a few articles citing expression in various tissues:
Cervix, Pubmed ID: 14702039
Kidney, Pubmed ID: 1337742, 8413205, 15489334
Boster Scientific Support
Answered: 2017-10-17
Question
Is this M00943-2 anti-PAX8 Rabbit Monoclonal antibody reactive to the isotypes of PAX8?
Verified Customer
Verified customer
Asked: 2017-07-19
Answer
The immunogen of M00943-2 anti-PAX8 Rabbit Monoclonal antibody is A synthesized peptide derived from human PAX8. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-19
Question
My question regarding product M00943-2, anti-PAX8 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Krishna
Verified customer
Asked: 2016-07-20
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00943-2 anti-PAX8 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-07-20
Question
Will anti-PAX8 Rabbit Monoclonal antibody M00943-2 work for IF with right lobe of thyroid gland?
C. Li
Verified customer
Asked: 2015-04-22
Answer
According to the expression profile of right lobe of thyroid gland, PAX8 is highly expressed in right lobe of thyroid gland. So, it is likely that anti-PAX8 Rabbit Monoclonal antibody M00943-2 will work for IF with right lobe of thyroid gland.
Boster Scientific Support
Answered: 2015-04-22
Question
I see that the anti-PAX8 Rabbit Monoclonal antibody M00943-2 works with IF, what is the protocol used to produce the result images on the product page?
O. Bhatt
Verified customer
Asked: 2014-03-06
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-03-06
Question
Does M00943-2 anti-PAX8 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Miller
Verified customer
Asked: 2013-02-15
Answer
As indicated on the product datasheet, M00943-2 anti-PAX8 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-02-15
