Product Info Summary
SKU: | M01153 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PBR TSPO Rabbit Monoclonal Antibody
SKU/Catalog Number
M01153
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PBR TSPO Rabbit Monoclonal Antibody catalog # M01153. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PBR TSPO Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01153)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DAG-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PBR
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01153 is reactive to TSPO in Human, Mouse
Applications
M01153 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
40 kDa
Calculated molecular weight
10.537kDa
Background of PBR
Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human colon, using PBR Antibody .
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)
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Western blot analysis of Calreticulin expression in (1) U87-MG cell lysate; (2) A431 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For TSPO (Source: Uniprot.org, NCBI)
Gene Name
TSPO
Full Name
Putative peripheral benzodiazepine receptor-related protein
Weight
10.537kDa
Alternative Names
benzodiazepine peripheral binding site; BZRPDBI; IBP; MBRPBS; mDRC; Mitochondrial benzodiazepine receptor; PBR; PBRBPBS; PBRS; peripheral benzodiazepine receptor; Peripheral-type benzodiazepine receptor; pk18; PKBSbenzodiazapine receptor (peripheral); PTBR; translocator protein (18kDa); translocator protein; TSPO TSPO BPBS, BZRP, DBI, IBP, MBR, PBR, PBS, PKBS, PTBR, mDRC, pk18 translocator protein translocator protein|benzodiazepine peripheral binding site|mitochondrial benzodiazepine receptor|peripheral-type benzodiazepine receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TSPO, check out the TSPO Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TSPO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PBR TSPO Rabbit Monoclonal Antibody (M01153)
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No publications found for M01153
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Customer Reviews
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Customer Q&As
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2 Customer Q&As for Anti-PBR TSPO Rabbit Monoclonal Antibody
Question
Can M01153 target dTSPO?
Verified customer
Asked: 2019-05-09
Answer
We haven't detected dTSPO using Anti-PBR TSPO Rabbit Monoclonal Antibody (M01153). The immunogen sequence is CRDNHGWHGGRRLPE.
Boster Scientific Support
Answered: 2019-05-10
Question
We are currently using anti-PBR Rabbit Monoclonal antibody M01153 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-26
Answer
The anti-PBR Rabbit Monoclonal antibody (M01153) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-26