Anti-PCBP2/hnRNP E2 Antibody Picoband® (monoclonal, 4B9C7)

PCBP2 antibody

Boster Bio Anti-PCBP2/hnRNP E2 Antibody Picoband® (monoclonal, 4B9C7) catalog # M02425. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: M02425
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, ICC, WB

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Product Name

Anti-PCBP2/hnRNP E2 Antibody Picoband® (monoclonal, 4B9C7)

View all PCBP2 Antibodies

SKU/Catalog Number

M02425

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PCBP2/hnRNP E2 Antibody Picoband® (monoclonal, 4B9C7) catalog # M02425. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PCBP2/hnRNP E2 Antibody Picoband® (monoclonal, 4B9C7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02425)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

4B9C7

Isotype

Mouse IgG2a

Immunogen

E.coli-derived human PCBP2/hnRNP E2 recombinant protein (Position: Q197-K276).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M02425 is reactive to PCBP2 in Human, Mouse, Rat

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

39 kDa

Calculated molecular weight

38.58kDa

Background of PCBP2

Poly(rC)-binding protein 2 is a protein that in humans is encoded by the PCBP2 gene. The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. This gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M02425 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human

Positive Control

WB: human T-47D whole cell, human HL-60 whole cell, human A549 whole cell, human HepG2 whole cell, rat brain tissue, mouse brain tissue
ICC/IF: Caco-2 cell
FCM: PC-3 cell

Validation Images & Assay Conditions

Gene/Protein Information For PCBP2 (Source: Uniprot.org, NCBI)

Gene Name

PCBP2

Full Name

Poly(rC)-binding protein 2

Weight

38.58kDa

Alternative Names

Peptidyl-prolyl cis-trans isomerase D; PPIase D PCBP2 HNRNPE2, HNRPE2, hnRNP-E2 poly(rC) binding protein 2 poly(rC)-binding protein 2|alpha-CP2|epididymis secretory sperm binding protein|heterogeneous nuclear ribonucleoprotein E2|heterogenous nuclear ribonucleoprotein E2|hnRNP E2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PCBP2, check out the PCBP2 Infographic

PCBP2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PCBP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M02425

100μg

$370
M02425-10ug

10μg sample (liquid)

$99
M02425-Biotin

100 μg Biotin conjugated

$570
M02425-Cy3

100 μg Cy3 conjugated

$570
M02425-Dylight488

100 μg Dylight488 conjugated

$570
M02425-Dylight550

100 μg Dylight550 conjugated

$570
M02425-Dylight594

100 μg Dylight594 conjugated

$570
M02425-FITC

100 μg FITC conjugated

$570
M02425-HRP

100 μg HRP conjugated

$570
M02425-APC

100 μg APC conjugated

$670
M02425-PE

100 μg PE conjugated

$670
M02425-iFluor647

100 μg iFluor647 conjugated

$670
M02425-carrier-free

Carrier Free

$370

More conjugates/formats

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M02425
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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