Product Info Summary
SKU: | A17592 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-PCDHGA2 Antibody Picoband™
SKU/Catalog Number
A17592
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PCDHGA2 Antibody Picoband™ catalog # A17592. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PCDHGA2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17592)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Clone Number
1B9
Isotype
IgG
Immunogen
E.coli-derived human PCDHGA2 recombinant protein (Position: R132-D444). Human PCDHGA2 shares 60.3% amino acid (aa) sequence identity with mouse PCDHGA2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A17592 is reactive to PCDHGA2 in Human, Mouse
Applications
A17592 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
90 kDa
Calculated molecular weight
101.484kDa
Background of PCDHGA2
This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PCDHGA2 using anti-PCDHGA2 antibody (A17592).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCDHGA2 antigen affinity purified polyclonal antibody (Catalog # A17592) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCDHGA2 at approximately 90 kDa. The expected band size for PCDHGA2 is at 90,101 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PCDHGA2 (Source: Uniprot.org, NCBI)
Gene Name
PCDHGA2
Full Name
Protocadherin gamma-A2
Weight
101.484kDa
Alternative Names
PCDH-GAMMA-A2; protocadherin gamma subfamily A, 2; protocadherin gamma-A2 PCDHGA2 PCDH-GAMMA-A2 protocadherin gamma subfamily A, 2 protocadherin gamma-A2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PCDHGA2, check out the PCDHGA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PCDHGA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PCDHGA2 Antibody Picoband™ (A17592)
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No publications found for A17592
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