Anti-PCF11 Antibody Picoband™

Product Name

Anti-PCF11 Antibody Picoband™

View all PCF11 Antibodies

SKU/Catalog Number

A05372-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PCF11 Antibody Picoband™ catalog # A05372-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PCF11 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05372-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human PCF11 recombinant protein (Position: N1214-V1555).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A05372-1 is reactive to PCF11 in Human, Mouse, Rat

Applications

A05372-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

200 kDa

Calculated molecular weight

173.05kDa

Background of PCF11

The protein encoded by this gene binds to CLP1 to form pre-mRNA cleavage factor IIm. The encoded protein is necessary for efficient Pol II transcription termination and may be involved in degradation of the 3' product of polyA site cleavage.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of PCF11 using anti-PCF11 antibody (A05372-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: rat testis tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCF11 antigen affinity purified polyclonal antibody (Catalog # A05372-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCF11 at approximately 200 kDa. The expected band size for PCF11 is at 173 kDa.

Gene/Protein Information For PCF11 (Source: Uniprot.org, NCBI)

Alternative Names

KIAA0824PCF11p homolog; PCF11, cleavage and polyadenylation factor II subunit, homolog (S. cerevisiae); PCF11, cleavage and polyadenylation factor II subunit, homolog; PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae); pre-mRNA cleavage complex 2 protein Pcf11; Pre-mRNA cleavage complex II protein Pcf11 PCF11 PCF11 cleavage and polyadenylation factor subunit pre-mRNA cleavage complex 2 protein Pcf11|PCF11, cleavage and polyadenylation factor subunit, homolog|PCF11p homolog|pre-mRNA cleavage complex II protein Pcf11

For more info on PCF11, check out the PCF11 Infographic

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In this infographic, you will see the following information for PCF11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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