Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2)

Product Name

Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2)

View all KRT1 Antibodies

SKU/Catalog Number

MA1081

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) catalog # MA1081. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1081)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2

Isotype

Mouse IgG2a/IgG1

Immunogen

mixture of several monoclonal cytokeratin clones.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1081 is reactive to KRT1 in Human, Rat

Applications

MA1081 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

45-60 kDa

Calculated molecular weight

66.039kDa

Background of KRT1

Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.1-0.5μg/ml, Human, rat, By Heat
Immunohistochemistry (Frozen Section), 0.1-0.5μg/ml, Human, rat, -
Western blot, 1-2μg/ml, Human, rat

Validation Images & Assay Conditions

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Figure 1. IHC analysis of PCK using anti-PCK antibody (MA1081).
PCK was detected in a paraffin-embedded section of Human Placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 0.5 μg/ml mouse anti-PCK Antibody (MA1081) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.

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Figure 2. Western blot analysis of PCK using anti-PCK antibody (MA1081).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human NCL-H460 whole cell lysates.
Lane 2: human Hela whole cell lysates.
Lane 3: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PCK antigen affinity purified monoclonal antibody (Catalog # MA1081) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PCK at approximately 45-60 kDa. The expected band size for PCK is at 66 kDa.

Gene/Protein Information For KRT1 (Source: Uniprot.org, NCBI)

Alternative Names

CK1; CK-1; cytokeratin 1; Cytokeratin-1; cytokeratin-1,67 kDa cytokeratin; EHK; EHK1; epidermolytic hyperkeratosis 1; EPPK; Hair alpha protein; K1; keratin 1; keratin, type II cytoskeletal 1; Keratin-1; KRT1A; KRTA; NEPPK; Type-II keratin Kb1 KRT1 CK1, EHK, EHK1, EPPK, K1A, NEPPK, KRT1 keratin 1 keratin, type II cytoskeletal 1|67 kDa cytokeratin|CK-1|cytokeratin 1|epidermolytic hyperkeratosis 1|hair alpha protein|keratin 1, type II|type-II keratin Kb1

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