This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Pcna antibody (M00125-6) performs well in IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Designing a Western blot for PCNA? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the PCNA Western Blot GuideBundle Save
Anti-PCNA Antibody (Monoclonal, 36P00)
Pcna antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | IHC, ICC, WB |
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.4-1μg/ml
Human, mouse, rat
Immunocytochemistry
1μg/ml
Human, mouse, rat, -
Immunohistochemistry (Frozen Section)
0.4-1μg/ml
Human, mouse, rat, -
Western blot
2μg/ml
Human, mouse, rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-PCNA Antibody (Monoclonal, 36P00)
View all PCNA Antibodies
View all PCNA Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
36P00
Isotype
Mouse IgG2a
Immunogen
Protein A fusion protein.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Calculated Molecular Weight
28.7 kDa
Background of Pcna
Proliferating cell nuclear antigen (PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA/cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.
Cite This Product
Anti-PCNA Antibody (Monoclonal, 36P00) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00125-6)
Review
Have you used Anti-PCNA Antibody (Monoclonal, 36P00)?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
2 Reviews For Anti-PCNA Antibody (Monoclonal, 36P00)
The PCNA antibody (Cat# M00125-6) showed clear and distinct nuclear positivity in HepG2-derived subcutaneous tumors, indicating reliable detection.
| SKU | M00125-6 |
|---|---|
| Application | Immunohistochemistry |
| Sample | HepG2 subcutaneous xenograft in nude mice |
| Sample Processing Description | HepG2 cells were expanded and then implanted subcutaneously into nude mice. After 2 weeks, tumors formed, which were excised, fixed in formalin for 48 hours, and processed for paraffin embedding and sectioning. |
| Other Reagents | Goat serum, DAB chromogen solution |
| Primary Antibody | PCNA Antibody (Monoclonal, PC 10) |
| Primary Incubation | 1:500, overnight at 4 ℃ |
| Secondary Antibody | Two-step IHC detection kit |
| Secondary Incubation | 30 min in 37℃ |
| Detection | Image system: Leica DM2500 |
| Results Summary | PCNA is a key marker for cell proliferation studies; however, besides being a proliferation marker, it is a core component of the DNA replication and repair complex. Its long half-life and involvement in DNA damage repair mean that PCNA positivity does not solely indicate cell proliferation but may also reflect cells responding to DNA damage. Combined with Ki67 staining, it can accurately indicate proliferative status. In our experiment, the staining was clear, with distinct nuclear positivity. |
Fengtong Wang, The First Affiliated Hospital of Xinjiang Medical University
Verified customer
Submitted 2026-03-31
Highly prone to positive signals, with precise localization of expression
| SKU | MA1083 |
|---|---|
| Application | Immunofluorenscence |
| Sample | Mouse MC-8 cells |
| Sample Processing Description | 4% paraformaldehyde for 15 minutes |
| Primary Antibody | Anti-PCNA Antibody (Monoclonal, PC 10) |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Blocking Agent | Goat serum |
| Secondary Antibody | DyLight 550-conjugated goat anti-rabbit antibody. |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Detection | Laser confocal microscopy |
| Results Summary | I will purchase Boster products again and recommend them to my classmates and colleagues. |
Guangyu Mei, Tongji University
Verified customer
Submitted 2025-10-17
Product Promise
We promise Anti-PCNA Antibody (Monoclonal, 36P00) performs excellently in relevant applications for IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00125-6
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 16, 2026