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Product Info Summary
| SKU: | A00085-3 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-PCSK9 Antibody (N-term)
SKU/Catalog Number
A00085-3
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-PCSK9 Antibody (N-term) (Catalog # A00085-3). Tested in Flow Cytometry, IHC-P, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PCSK9 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00085-3)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This PCSK9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-183 amino acids from the N-terminal region of human PCSK9.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00085-3 is reactive to PCSK9 in Human
Calculated molecular weight
74.3 kDa
Background of PCSK9
PCSK9 is a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. The protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00085-3 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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Western blot analysis of lysate from SW480 cell line, using PCSK9 Antibody (N-term). A00085-3 was diluted at 1:2000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Western blot analysis of lysate from SW480 cell line, using PCSK9 Antibody (N-term). A00085-3 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Western blot analysis of lysate from Hela cell line, using PCSK9 Antibody (N-term). A00085-3 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Western blot analysis of PCSK9 Antibody (N-term) in Jurkat cell line lysates (35ug/lane). PCSK9 (arrow) was detected using the purified Pab.
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Immunohistochemical analysis of paraffin-embedded H. colorectal carcinoma section using PCSK9 Antibody (N-term). A00085-3 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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PCSK9 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PCSK9 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Flow cytometric analysis of A431 cells using PCSK9 Antibody (N-term) (green, Cat#A00085-3) compared to an isotype control of rabbit IgG(blue). A00085-3 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Specific Publications For Anti-PCSK9 Antibody (N-term) (A00085-3)
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