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Product Info Summary
| SKU: | A00178-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-PD-1 PDCD1 Antibody
SKU/Catalog Number
A00178-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PD-1 PDCD1 Antibody (Catalog # A00178-2). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PD-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PD-1 PDCD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00178-2)
Host
Rabbit
Contents
PD-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-PD-1 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human PD-1. The immunogen is located within amino acids 210-260 of PD-1.
Reactive Species
A00178-2 is reactive to PDCD1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
31.6 kDa
Background of PDCD1
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00178-2 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-4 μg/mL; IHC: 2.5-5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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KO Validation in HeLa Cells
Loading: 10 μg of HeLa WT cell lysates or PD-1 KO cell lysates. Antibodies: PD-1, A00178-2 (4 μg/mL) and beta-actin 3779 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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KD Validation in HeLa Cells
Loading: 10 μg of HeLa WT cell lysates or PD-1 KD cell lysates. Antibodies: PD-1, A00178-2 (4 μg/mL) and beta-actin 3779 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Human and Mouse Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: PD-1 A00178-2 (4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in THP-1 Cell Lysate in the (A) absence and (B) presence of blocking peptide
Loading: 15 μg of lysates per lane.
Antibodies: PD-1, A00178-2 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Rat Thymus Cell Lysate
Loading: 15 μg of lysates per lane.
Antibodies: PD-1 A00178-2 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunohistochemistry Validation of PD-1 in Human Tonsil Tissue
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD-1 antibody (A00178-2) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of PD-1 in Human Tonsil Tissue
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD-1 antibody (A00178-2) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of PD-1 in Human Brain Tissue
Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-PD-1 antibody (A00178-2) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Specific Publications For Anti-PD-1 PDCD1 Antibody (A00178-2)
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