Anti-PD-L1/B7-H1/CD274 Antibody Picoband™

Boster Bio Anti-PD-L1/B7-H1/CD274 Antibody Picoband™ catalog # A00109. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A00109
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-PD-L1/B7-H1/CD274 Antibody Picoband™

See all PD-L1 products

SKU/Catalog Number

A00109

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PD-L1/B7-H1/CD274 Antibody Picoband™ catalog # A00109. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PD-L1/B7-H1/CD274 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00109)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human PD-L1/B7-H1 (41-69aa KFPVEKQLDLAALIVYWEMEDKNIIQFVH), different from the related mouse sequence by ten amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00109 is reactive to CD274 in Human

Applications

A00109 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CD274 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9NZQ7

Gene ID

29126

Gene Name

CD274

Full Name

Programmed cell death 1 ligand 1

Weight

33.275kDa

Superfamily

immunoglobulin superfamily

Alternative Names

B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1 CD274 B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1 CD274 molecule programmed cell death 1 ligand 1|B7 homolog 1|CD274 antigen|PDCD1 ligand 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD274, check out the CD274 Infographic

CD274 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD274: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A00109

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15 Customer Q&As for Anti-PD-L1/B7-H1/CD274 Antibody Picoband™

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for gastric carcinoma using anti-PD-L1/B7-H1/CD274 antibody A00109. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-03-25

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-25

Question

I would like to test anti-PD-L1/B7-H1/CD274 antibody A00109 on human gastric carcinoma for research purposes, then I may be interested in using anti-PD-L1/B7-H1/CD274 antibody A00109 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-30

Answer

The products we sell, including anti-PD-L1/B7-H1/CD274 antibody A00109, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-30

Question

Will A00109 anti-PD-L1/B7-H1/CD274 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-12-27

Answer

You can see on the product datasheet, A00109 anti-PD-L1/B7-H1/CD274 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-27

Question

See below the WB image, lot number and protocol we used for gastric carcinoma using anti-PD-L1/B7-H1/CD274 antibody A00109. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-10

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-10

Question

We are currently using anti-PD-L1/B7-H1/CD274 antibody A00109 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

The anti-PD-L1/B7-H1/CD274 antibody (A00109) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-29

Question

Is a blocking peptide available for product anti-PD-L1/B7-H1/CD274 antibody (A00109)?

Verified Customer

Verified customer

Asked: 2019-10-30

Answer

We do provide the blocking peptide for product anti-PD-L1/B7-H1/CD274 antibody (A00109). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-10-30

Question

I see that the anti-PD-L1/B7-H1/CD274 antibody A00109 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-28

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-28

Question

We were happy with the WB result of your anti-PD-L1/B7-H1/CD274 antibody. However we have seen positive staining in chorionic villus cell membrane using this antibody. Is that expected? Could you tell me where is CD274 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-25

Answer

From what I have seen in literature, chorionic villus does express CD274. Generally CD274 expresses in cell membrane. Regarding which tissues have CD274 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Placenta, Pubmed ID: 11015443, 14702039

Boster Scientific Support

Answered: 2019-10-25

Question

Do you have a BSA free version of anti-PD-L1/B7-H1/CD274 antibody A00109 available?

Verified Customer

Verified customer

Asked: 2019-09-06

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PD-L1/B7-H1/CD274 antibody A00109 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-06

Question

We have been able to see staining in human liver. Any tips? Is anti-PD-L1/B7-H1/CD274 antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2018-04-19

Answer

From what I have seen in literature liver does express CD274. From what I have seen in Uniprot.org, CD274 is expressed in chorionic villus, placenta, gastric carcinoma, lung, liver, among other tissues. Regarding which tissues have CD274 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Placenta, Pubmed ID: 11015443, 14702039

Boster Scientific Support

Answered: 2018-04-19

Question

Does anti-PD-L1/B7-H1/CD274 antibody A00109 work for WB with gastric carcinoma?

Verified Customer

Verified customer

Asked: 2017-12-11

Answer

According to the expression profile of gastric carcinoma, CD274 is highly expressed in gastric carcinoma. So, it is likely that anti-PD-L1/B7-H1/CD274 antibody A00109 will work for WB with gastric carcinoma.

Boster Scientific Support

Answered: 2017-12-11

Question

I was wanting to use your anti-PD-L1/B7-H1/CD274 antibody for WB for human gastric carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human gastric carcinoma identification?

Verified Customer

Verified customer

Asked: 2017-10-02

Answer

It shows on the product datasheet, A00109 anti-PD-L1/B7-H1/CD274 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human gastric carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-10-02

Question

I am interested in using your anti-PD-L1/B7-H1/CD274 antibody for negative regulation of interleukin-10 production studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-09-14

Answer

Thank you for your inquiry. This A00109 anti-PD-L1/B7-H1/CD274 antibody is validated on hela whole cell lysates. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-09-14

Question

Can you help my question with product A00109, anti-PD-L1/B7-H1/CD274 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Z. Miller

Verified customer

Asked: 2016-07-06

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00109 anti-PD-L1/B7-H1/CD274 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-07-06

Question

Is this A00109 anti-PD-L1/B7-H1/CD274 antibody reactive to the isotypes of CD274?

D. Johnson

Verified customer

Asked: 2015-03-30

Answer

The immunogen of A00109 anti-PD-L1/B7-H1/CD274 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human PD-L1/B7-H1 (41-69aa KFPVEKQLDLAALIVYWEMEDKNIIQFVH), different from the related mouse sequence by ten amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2015-03-30

Size

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A00109
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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