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- Table of Contents
CD274 antibody (A00109-1) performs well in ELISA, Flow Cytometry, IF, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry Validation of PD-L1
Overlay histogram showing A-20 cells stained with A00109-1 (red line, 1μg/1x106 cells). 1 h incubation at 4˚C in 2% FBS/PBS. Followed by secondary antibody 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 1 h 4˚C. Isotype control antibody (Green line) was mouse IgG1 (1μg/1x106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Overlay histogram showing A-20 cells stained with A00109-1 (red line, 1μg/1x106 cells). 1 h incubation at 4˚C in 2% FBS/PBS. Followed by secondary antibody 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 1 h 4˚C. Isotype control antibody (Green line) was mouse IgG1 (1μg/1x106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Anti-PD-L1 CD274 Antibody
CD274 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
WB: 1-2 μg/mL; IHC-P: 2.5-5 μg/mL; IF: 20 μg/mL; Flow Cyt: 0.5 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human and rat samples; Immunofluorescence in human and rat samples and Flow Cytometry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-PD-L1 CD274 Antibody
View all CD274 Antibodies
View all CD274 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
PD-L1 antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
PD-L1 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-PD-L1 antibody was raised against a peptide corresponding to 17 amino acids near the center of human PD-L1 isoform 1. The immunogen is located within amino acids 60 - 110 of PD-L1.
Cross-Reactivity
PD-L1 antibody has no cross-reactivity to PD-L2.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
33.3 kDa
Background of CD274
PD-L1 plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, PD-L1 modulates the activation threshold of T-cells and limits T-cell effector response (1). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (2,3). Through a yet unknown activating receptor, it may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (4).
Cite This Product
Anti-PD-L1 CD274 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00109-1)
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Product Promise
We promise Anti-PD-L1 CD274 Antibody performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00109-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026