SKU M00178-1
Size 100ug/vial
Reactivity Human
Clonality Monoclonal
Clone Number BEG-16
Host Rabbit
Applications IHC

Overview

Product Name Anti-PD1 Rabbit Monoclonal Antibody
SKU/Catalog Number M00178-1
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100ug/vial
Description Anti-PD1 Rabbit Monoclonal Antibody tested for Flow Cytometry, IHC in Human
Cite This Product Anti-PD1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00178-1)
Host Rabbit
Contents/Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Immunogen A synthesized peptide derived from human PD1
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
IHC 1:50-1:200
Flow Cytometry 1:50

Images And Assay Conditions

/M/0/M00178 1 PDCD1 primary antibodies IHC testing 2.jpg

Immunohistochemical analysis of paraffin-embedded human tonsil, using PD1 Antibody (M00178-1)
PDCD1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PDCD1 Antibody (M00178-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q15116
Gene Name PDCD1
Protein Name Programmed cell death protein 1
Alternative Names Programmed cell death protein 1;Protein PD-1;hPD-1;CD279;PDCD1;PD1;
Subcellular Localization Membrane; Single-pass type I membrane protein.
Molecular Weight 31647 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen- specific manner. Possible cell death inducer, in association with other factors. .
Research Areas Protein Phosphorylation, Ser / Thr Kinases, Signal Transduction

*You can search these to find other products in these research areas.

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Order Product (M00178-1)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
100ug $299
100ug+Free HRP Secondary BA1054 $299
100ug+Free Biotin Secondary BA1003 $299
30ug sample size $99

USD $299

Ships in 7-10 business days.

Troubleshooting

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