Anti-PD1 Rabbit Monoclonal Antibody
|Product Name||Anti-PD1 Rabbit Monoclonal Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.|
|Description||Anti-PD1 Rabbit Monoclonal Antibody tested for Flow Cytometry, IHC in Human|
|Cite This Product||Anti-PD1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00178-1)|
|Contents/Buffer||Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|Immunogen||A synthesized peptide derived from human PD1|
Assay Dilutions Overview
Flow Cytometry 1:50
Images And Assay Conditions
Immunohistochemical analysis of paraffin-embedded human tonsil, using PD1 Antibody (M00178-1)
PDCD1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PDCD1 Antibody (M00178-1)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info (Source: Uniprot.org)
|Protein Name||Programmed cell death protein 1|
|Alternative Names||Programmed cell death protein 1;Protein PD-1;hPD-1;CD279;PDCD1;PD1;|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Molecular Weight||31647 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen- specific manner. Possible cell death inducer, in association with other factors. .|
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