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Product Info Summary
| SKU: | M03295-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-PDCD1LG2 Antibody (N-term)
SKU/Catalog Number
M03295-1
Size
50 µl
Description
Boster Bio Anti-PDCD1LG2 Antibody (N-term) (Catalog # M03295-1). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PDCD1LG2 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03295-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
2011CT241.19.54
Isotype
IgG1,κ
Immunogen
This PDCD1LG2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 40-75 amino acids from the N-terminal region of human PDCD1LG2.
Reactive Species
M03295-1 is reactive to PDCD1LG2 in Human
Calculated molecular weight
31.0 kDa
Background of PDCD1LG2
Involved in the costimulatory signal, essential for T- cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03295-1 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-PDCD1LG2 Antibody (N-term) at 1:4000 dilution
Lane 1: Non-transfected 293T whole cell lysate
Lane 2: Transfected PD-L2-transfected 293T whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 31 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of PDL-2 in untransfected(left) or transfected(right) with 293T cell sections. Cell was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:25) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-PDCD1LG2 Antibody (N-term) (M03295-1)
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