This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
PDGFA antibody (A02257-1) performs well in ELISA, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
Filter:
IF analysis of PDGF AA/PDGFA using anti-PDGF AA/PDGFA antibody (A02257-1).
PDGF AA/PDGFA was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PDGF AA/PDGFA Antibody (A02257-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
PDGF AA/PDGFA was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PDGF AA/PDGFA Antibody (A02257-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Bundle Save
Anti-PDGF AA/PDGFA Antibody Picoband®
PDGFA antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, ICC, WB |
Tested Applications
PDGFA antibody was positively detected by WB in:
human Hacat whole cell, rat PC-12 whole cell, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PDGFA antibody was positively detected by ICC/IF in:
SiHa cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
ELISA
Human
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Dog
Predicted
Predicted
Rabbit
Predicted
Predicted
Product Information
Product Name
Anti-PDGF AA/PDGFA Antibody Picoband®
View all PDGFA Antibodies
View all PDGFA Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PDGF AA/PDGFA recombinant protein (Position: S87-T211).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
24 kDa
Calculated Molecular Weight
24.0 kDa
Background of PDGFA
Platelet-derived growth factor subunit A is a protein that in humans is encoded by the PDGFA gene. This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants.
Cite This Product
Anti-PDGF AA/PDGFA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02257-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
95%
High Homology
Rat
95%
High Homology
Dog
91%
High Homology
Rabbit
90%
High Homology
Cow
89%
Moderate Homology
Sheep
89%
Moderate Homology
Monkey
85%
Moderate Homology
Zebrafish
63%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PDGFA antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for PDGFA antibody
Publications
Application:
Species:
Ginsenoside Rh2 Inhibits Angiogenesis in Prostate Cancer by Targeting CNNM1
Published: 2019-04-01
Journal: JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
Application: WB
Impact Factor: 1.09
Species: Human
PMID: 30486934
The effects of low-molecular-weight heparin on lung and pulmonary artery injuries in acute pulmonary embolism rat model via platelet-derived growth factor-β
Published: 2017-04-28
Journal: SAUDI PHARMACEUTICAL JOURNAL
Application: IHC
Impact Factor: 2.30
Species: Rat
PMID: 28579892
Effects of Monocyte Chemotactic Protein-1 and Nuclear Factor of Kappa B Pathway in Rejection of Cardiac Allograft in Rat
Published: 2015-08-19
Journal: TRANSPLANTATION PROCEEDINGS
Application: IHC
Impact Factor: 0.98
Species: Rat
PMID: 26293090
Review
Have you used Anti-PDGF AA/PDGFA Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-PDGF AA/PDGFA Antibody Picoband® performs excellently in relevant applications for ELISA, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A02257-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026