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- Table of Contents
PDGFA · Western blot design guide
PDGFA Western Blot Planning Guide
Plan a PDGFA Western blot around the catalog-observed 24 kDa band, image-backed A02257-1 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
PDGFA Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 24 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Cervix | |
| Negative control | Bone marrow |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 24 kDa |
| ⓘ | Localization | Secreted |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated PDGFA Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Adipose tissue |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, short transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A02257-1 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected PDGFA Western Blot Band Size?
Use the product-observed 24 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 24 kDa | Matches the authoritative product WB observation. |
| 24 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected PDGFA appearancePlan around 24 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 24 kDa; use this as the primary experimental expectation. |
| Calculated mass | 24 kDa from UniProt P04085; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A02257-1. |
| Situation | Likely cause | Next action |
|---|---|---|
| 24 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing PDGFA band
A02257-1 · PDGFA
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PDGFA Western blot
🧪Use Cervix as the first positive-control candidate and Bone marrow as the HPA Not detected negative candidate.
Positive control: Cervix (High)
Negative control: Bone marrow (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for PDGFA
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cervix | Reported tissue cells | High | Protein (HPA) | HPA → |
| Breast | Reported tissue cells | High | Protein (HPA) | HPA → |
| Cerebellum | Reported tissue cells | High | Protein (HPA) | HPA → |
| Adipose tissue | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced PDGFA Western Blot Tips
Deeper troubleshooting and optimisation questions for PDGFA, answered from its protein features.
Which band should guide the blot?
Use 24 kDa, the observation attached to the authoritative A02257-1 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 24 kDa expectation.
Which positive control should I start with?
Start with Cervix, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Bone marrow as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for PDGFA Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A02257-1 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
PDGFA Western Blot Reagents
Human/Mouse/Rat-reactive PDGFA Western blot reagents with authoritative product imagery.
Real WB data
Anti-PDGF AA/PDGFA Antibody Picoband®
Cat # A02257-1
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P04085
- Human Protein Atlas — PDGFA
- A02257-1 product record
- PMC11662046 — KLF5 silencing restrains proliferation, invasion, migration and angiogenesis of gallbladder carcinoma cells by transcriptional regulation of PDGFA (Journal of cancer research and clinical oncology, 2024)
- PMC13045304 — Microglial GPR35 Ameliorates Epileptogenesis and Neuroinflammation via PDGFA Domain 2 Signaling (Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026)
- PMC3337953 — MUC1 regulates PDGFA expression during pancreatic cancer progression (Oncogene, 2012)
