Anti-PDGF AA/PDGFA Antibody Picoband™

Product Name

Anti-PDGF AA/PDGFA Antibody Picoband™

View all Pdgfa Antibodies

SKU/Catalog Number

A02257-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PDGF AA/PDGFA Antibody Picoband™ catalog # A02257-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PDGF AA/PDGFA Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02257-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PDGF AA/PDGFA recombinant protein (Position: S87-T211).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02257-1 is reactive to PDGFA in Human, Mouse, Rat

Applications

A02257-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

24 kDa

Calculated molecular weight

24.043kDa

Background of Pdgfa

Platelet-derived growth factor subunit A is a protein that in humans is encoded by the PDGFA gene. This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PDGF AA/PDGFA using anti-PDGF AA/PDGFA antibody (A02257-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hacat whole cell lysates,
Lane 2: rat PC-12 whole cell lysates,
Lane 3: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGF AA/PDGFA antigen affinity purified polyclonal antibody (Catalog # A02257-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDGF AA/PDGFA at approximately 24 kDa. The expected band size for PDGF AA/PDGFA is at 24 kDa.

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Figure 2. IF analysis of PDGF AA/PDGFA using anti-PDGF AA/PDGFA antibody (A02257-1).
PDGF AA/PDGFA was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PDGF AA/PDGFA Antibody (A02257-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For PDGFA (Source: Uniprot.org, NCBI)

Alternative Names

PDGFAA; PDGF-AA PDGFA PDGF-A, PDGF1 platelet derived growth factor subunit A platelet-derived growth factor subunit A|PDGF A-chain|PDGF subunit A|platelet-derived growth factor A-chain|platelet-derived growth factor alpha chain|platelet-derived growth factor alpha polypeptide

For more info on PDGFA, check out the PDGFA Infographic

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In this infographic, you will see the following information for PDGFA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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