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Product Info Summary
| SKU: | PB9311 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PDGF beta/PDGFB Antibody Picoband®
SKU/Catalog Number
PB9311
PB0346 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PDGF beta/PDGFB Antibody Picoband® catalog # PB9311. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDGF beta/PDGFB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9311)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PDGF beta recombinant protein (Position: S82-T190). Human PDGF beta shares 89% amino acid (aa) sequence identity with both mouse and rat PDGF beta.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9311 is reactive to PDGFB in Human, Mouse, Rat
Observed Molecular Weight
13 kDa
Calculated molecular weight
27.3 kDa
Background of PDGFB
Platelet-derived growth factor subunit B is a protein that in humans is encoded by the PDGFB gene. The protein encoded by this gene is a member of the platelet-derived growth factor family. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. This gene is mapped to 22q13.1. Growth factor plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. This gene plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9311 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Cardiac Muscle Tissue, Rat Brain Tissue, Mouse Cardiac Muscle Tissue, HELA Whole Cell
Validation Images & Assay Conditions
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Anti-PDGF beta Picoband antibody, PB9311, Western blotting
All lanes: Anti PDGF beta (PB9311) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 13KD
Specific Publications For Anti-PDGF beta/PDGFB Antibody Picoband® (PB9311)
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4 Customer Q&As for Anti-PDGF beta/PDGFB Antibody Picoband®
Question
Will anti-PDGF beta/PDGFB antibody PB9311 work for WB with pancreas testis?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
According to the expression profile of pancreas testis, PDGFB is highly expressed in pancreas testis. So, it is likely that anti-PDGF beta/PDGFB antibody PB9311 will work for WB with pancreas testis.
Boster Scientific Support
Answered: 2019-11-14
Question
Is this PB9311 anti-PDGF beta/PDGFB antibody reactive to the isotypes of PDGFB?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
The immunogen of PB9311 anti-PDGF beta/PDGFB antibody is E.coli-derived human PDGF beta recombinant protein (Position: S82-T190). Human PDGF beta shares 89% amino acid (aa) sequence identity with both mouse and rat PDGF beta. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-15
Question
My question regarding product PB9311, anti-PDGF beta/PDGFB antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Gonzalez
Verified customer
Asked: 2018-05-02
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9311 anti-PDGF beta/PDGFB antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-02
Question
We are currently using anti-PDGF beta/PDGFB antibody PB9311 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-10
Answer
The anti-PDGF beta/PDGFB antibody (PB9311) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-10
