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- Table of Contents
PDGFB antibody (PB9311) performs well in WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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Anti-PDGF beta Picoband antibody, PB9311, Western blotting
All lanes: Anti PDGF beta (PB9311) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 13KD
All lanes: Anti PDGF beta (PB9311) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 13KD
Designing a Western blot for PDGFB? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the PDGFB Western Blot GuideBundle Save
Anti-PDGF beta/PDGFB Antibody Picoband®
PDGFB antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | WB |
Tested Applications
PDGFB antibody was positively detected by WB in:
Rat Cardiac Muscle Tissue, Rat Brain Tissue, Mouse Cardiac Muscle Tissue, HELA Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
Rat
Tested
Mouse
Tested
Human
Predicted
Monkey
Predicted
Dog
Predicted
Rabbit
Predicted
Product Information
Product Name
Anti-PDGF beta/PDGFB Antibody Picoband®
View all PDGFB Antibodies
View all PDGFB Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PDGF beta recombinant protein (Position: S82-T190). Human PDGF beta shares 89% amino acid (aa) sequence identity with both mouse and rat PDGF beta.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
13 kDa
Calculated Molecular Weight
27.3 kDa
Background of PDGFB
Platelet-derived growth factor subunit B is a protein that in humans is encoded by the PDGFB gene. The protein encoded by this gene is a member of the platelet-derived growth factor family. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. This gene is mapped to 22q13.1. Growth factor plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. This gene plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.
Cite This Product
Anti-PDGF beta/PDGFB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9311)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Dog
96%
High Homology
Rabbit
92%
High Homology
Mouse
90%
High Homology
Rat
89%
Moderate Homology
Cow
89%
Moderate Homology
Sheep
88%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PDGFB antibody
Publications
Application:
Species:
Chemiluminescent detection of platelet derived growth factor-BB based on sandwich label-free aptasensor and biotin–streptavidin strategy
Published: 2022-05-26
Journal: JOURNAL OF IMMUNOLOGICAL METHODS
Impact Factor: 2.29
PMID: 35644254
Protective effects of glutamine in a rat model of endotoxemia
Published: 2012-07-26
Journal: Molecular Medicine Reports
Application: WB,IHC
Impact Factor: 0.42
Species: Rat
PMID: 22842774
Electrochemical immunosensor based on electron transfer mediated by graphene oxide initiated silver enhancement
Published: 2011-06-21
Journal: BIOSENSORS & BIOELECTRONICS
Impact Factor: 5.36
Species: Immunosensor
PMID: 21741229
Investigation of the protective effect of erythropoietin on spinal cord injury in rats
Published: 2011-06-14
Journal: Experimental and Therapeutic Medicine
Application: IHC
Impact Factor: 0.00
Species: Rat
PMID: 22977585
Runx3 suppresses gastric cancer metastasis through inactivation of MMP9 by upregulation of TIMP-1
Published: 2010-12-02
Journal: INTERNATIONAL JOURNAL OF CANCER
Impact Factor: 4.72
PMID: 21128246
Mechanisms of inhibiting proliferation of vascular smooth muscle cells by serum of rats treated with Dahuang Zhechong pill
Published: 2009-04-11
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: ICC
Impact Factor: 2.26
Species: Rat
PMID: 19527826
Electrochemical immunosensor of platelet-derived growth factor with aptamer-primed polymerase amplification
Published: 2008-07-16
Journal: ANALYTICAL BIOCHEMISTRY
Impact Factor: 3.00
Species: Material
PMID: 18675245
Aptamer-Based Rolling Circle Amplification: A Platform for Electrochemical Detection of Protein
Published: 2007-08-28
Journal: ANALYTICAL CHEMISTRY
Impact Factor: 5.65
PMID: 17722881
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Product Promise
We promise Anti-PDGF beta/PDGFB Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9311
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 23, 2026