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Product Info Summary
| SKU: | M02335-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-PDIA1 Antibody
SKU/Catalog Number
M02335-1
Size
50 µl
Description
Boster Bio Anti-PDIA1 Antibody (Catalog # M02335-1). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PDIA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02335-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1530CT836.11.53
Isotype
IgG1,k
Immunogen
This PDIA1 antibody is generated from a mouse immunized with a recombinant protein of human PDIA1.
Reactive Species
M02335-1 is reactive to P4HB in Human
Calculated molecular weight
57.1 kDa
Background of P4HB
This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02335-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
Validation Images & Assay Conditions
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All lanes : Anti-PDIA1 Antibody at 1:4000 dilution
Lane 1: Hela whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: PANC-1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 57 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-PDIA1 Antibody (M02335-1)
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